A SENSITIVE AND SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DOXORUBICIN AND ITS METABOLITES IN PLASMA
A SENSITIVE AND SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DOXORUBICIN AND ITS METABOLITES IN PLASMA
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DOI：
10.1097/00007691-199310000-00016
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发表时间：
1993-10-01
影响因子：
2.5
通讯作者：
SLORDAL, L
中科院分区：
文献类型：
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作者：
ANDERSEN, A;WARREN, DJ;SLORDAL, L
建立了一种测定血浆中阿霉素及其代谢产物的高效液相色谱（HPLC）法。用硫酸锌和甲醇沉淀后，在20分钟内通过从C18反相支持物等度洗脱分离样品，并通过内源性荧光定量。浓度范围为5 - 1，000 nM的多柔比星、多柔比星酚、多柔比星酮、多柔比星烯醇酮和7-脱氧多柔比星烯醇酮的回收率为80- 110%，而7-脱氧多柔比星酮的回收率约为60%。在5 nM的浓度下，每种化合物的运行内和日间变异系数为
A sensitive high-performance liquid chromatographic (HPLC) method for the measurement of doxorubicin and its metabolites in plasma is described. After precipitation with zinc sulphate and methanol, samples are resolved by isocratic elution from a C18 reverse phase support within 20 min and quantified by endogenous fluorescence. Recoveries over a concentration range from 5 to 1,000 nM of doxorubicin, doxorubicinol, doxorubicinone, doxorubicinolone, and 7-deoxydoxorubicinolone were 80-110%, while recovery for 7-deoxydoxorubicinone was approximately 60%. At concentrations of 5 nM, within-run and between-day coefficients of variation for each compound were