Separately developing axonal uptake of 5-hydroxytryptamine and norepinephrine in the fetal ileum of the rabbit
Separately developing axonal uptake of 5-hydroxytryptamine and norepinephrine in the fetal ileum of the rabbit
复制标题
兔胎儿回肠中单独发育的轴突摄取 5-羟色胺和去甲肾上腺素
DOI：
10.1016/0006-8993(76)90360-7
复制
发表时间：
1976
期刊：
影响因子：
2.9
通讯作者：
M. Gershon
中科院分区：
文献类型：
--
作者：
T. P. Rothman;L. Ross;M. Gershon
本文研究了成年和胎兔回肠对5-羟色胺（5-HT）的摄取。成年肌间神经丛在氚标记的5-HT孵育时积累氚。然而，除了标记的5-HT，氚标记的5-羟基吲哚乙酸，当单胺氧化酶（MAO）被抑制，5-HT-O-葡糖苷酸被发现在组织中。可以定义两种亲和力不同的摄取过程。只有高亲和力过程是可饱和的。胎儿回肠采取了氚标记的5-HT，但葡萄糖醛酸化没有发生时，单胺氧化酶被抑制。胎儿回肠对氚化5-HT的摄取是一个单一的、饱和的、温度敏感的过程（Q10在27 - 37 °C= 2.4），该过程被哇巴因抑制。这与成人组织中发现的高亲和力摄取相同。这种特异性的高亲和力摄取早在妊娠第16天就可以发现，在去甲肾上腺素（NE）摄取开始前5-8天。光镜和电镜放射自显影显示，5-HT的摄取主要是进入轴突和一个特征性的结构称为扩大的过程，无论是在肌间神经丛。两者都含有致密核心囊泡。直到24天后，轴突才被氚标记，并且扩展的过程从未被氚标记。这项研究表明，摄取5-HT是一个独特的系统的轴突在哺乳动物肌间神经丛的肾上腺素能轴突发育之前，在个体发育的属性。
Uptake of 5-hydroxytryptamine (5-HT) by adult and fetal rabbit's ileum was studied. The adult myenteric plexus accumulated tritium when incubated with tritiated 5-HT. However, in addition to labeled 5-HT, tritiated 5-hydroxyindole acetic acid and, when monoamine oxidase (MAO) was inhibited, 5-HT-o-glucuronide were found in the tissue. Two uptake processes differing in affinity could be defined. Only the high affinity process was saturable. Fetal ileum took up tritiated 5-HT but glucuronidation did not occur when MAO was inhibited. The uptake of tritiated 5-HT by the fetal ileum was due to a single, saturable, temperature sensitive (Q10at 27−37 °C= 2.4) process inhibited by ouabain. It was identical to the high affinity uptake found in adult tissue. This specific high affinity uptake could be found as early as the 16th day of gestation, 5–8 days before uptake of norepinephrine (NE) begins. Light and electron microscopic radioautography revealed that the uptake of 5-HT was primarily into axons and a characteristic structure called the expanded process, both in the myenteric plexus. Both contained dense-cored vesicles. Axons were not labeled by tritiated NE until after 24 days and the expanded process was never labeled by tritiated NE. This study shows that uptake of 5-HT is a property of a distinct system of axons in the mammalian myenteric plexus which develops prior to adrenergic axons during ontogeny.