ROX1, a gene induced by rolB, is involved in procambial cell proliferation and xylem differentiation in tobacco stamen.
ROX1, a gene induced by rolB, is involved in procambial cell proliferation and xylem differentiation in tobacco stamen.
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DOI：
10.1111/j.1365-313x.2006.02934.x
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发表时间：
2006-11
期刊：
影响因子：
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通讯作者：
V. Cecchetti;M. Altamura;G. Serino;M. Pomponi;G. Falasca;P. Costantino;M. Cardarelli
中科院分区：
文献类型：
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作者：
V. Cecchetti;M. Altamura;G. Serino;M. Pomponi;G. Falasca;P. Costantino;M. Cardarelli
发根农杆菌癌基因rolB模拟生长素的作用，因为它增加了转化细胞对这种激素的敏感性。在这里，我们分离烟草基因，ROX1，作用于rolB的下游。我们发现，由于35S驱动的ROX1反义构建体的表达，ROX1 mRNA水平降低的植物具有比正常更长的雄蕊和雌蕊的花，因为细胞数量增加。rolB在花药中的局部表达导致ROX1的过表达和雄蕊的生长减少，这是由于细胞数量减少。此外，反义植物的较长雄蕊在侧束中表现出延迟的木质部分化，主要是花药和花丝之间的连接区域，而ROX1过表达植物的较短雄蕊在相同组织中表现出木质部细胞的早熟分化。ROX1在雄蕊中的表达在雄蕊生长的早期达到高峰，并且ROX1 mRNA主要定位于花药原形成层细胞。ROX1的序列与许多植物基因共享一个保守元件，包括参与木质部分化的TED3。这些结果表明ROX1在雄蕊发育过程中原形成层细胞增殖和木质部分化之间的平衡中起作用。
The Agrobacterium rhizogenes oncogene rolB mimics the effects of auxin in that it increases the sensitivity of transformed cells to this hormone. Here we isolated a tobacco gene, ROX1, acting downstream of rolB. We show that plants with reduced levels of ROX1 mRNA, due to the expression of a 35S-driven ROX1-antisense construct, have flowers with stamens and pistils longer than normal because of an increased number of cells. Localized expression of rolB in anthers results in overexpression of ROX1 and reduced growth of stamens, due to a reduced number of cells. In addition, the longer stamens of antisense plants show a delayed xylem differentiation in the lateral bundles, primarily of the junction region between anther and filament, while the shorter stamens of ROX1-overexpressing plants show a precocious differentiation of xylem cells in the same tissues. Expression of ROX1 in stamens peaks at early stages of stamen growth, and ROX1 mRNA is localized mostly in anther procambial cells. The sequence of ROX1 shares a conserved element with a number of plant genes, including TED3, which is involved in xylem differentiation. These results point to a role of ROX1 in the balance between proliferation of procambial cells and xylem differentiation during stamen development.