Identification of the major cottontail rabbit papillomavirus late RNA cap site and mapping and quantitation of an E2 and minor E6 coding mRNA in papillomas and carcinomas.
Identification of the major cottontail rabbit papillomavirus late RNA cap site and mapping and quantitation of an E2 and minor E6 coding mRNA in papillomas and carcinomas.
复制标题
乳头状瘤和癌中主要棉尾兔乳头瘤病毒晚期 RNA 帽位点的鉴定以及 E2 和次要 E6 编码 mRNA 的定位和定量。
DOI：
10.1016/0042-6822(87)90470-3
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发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Nasseri,M
中科院分区：
文献类型：
--
作者：
Wettstein,FO;Barbosa,MS;Nasseri,M
用引物延伸法对棉尾兔乳头瘤病毒（CRPV）的2.6-kb和4.8-kb主要晚期转录物的衣壳蛋白进行了定位。两种RNA共有的约300个核苷酸的前导外显子位于早期转录物的衣壳位点上游的基因组非翻译区。与之前都是TATA盒的早期帽位点相反，在晚期帽位点上游30个核苷酸处不存在这样的序列。这些数据表明，从早期转录到晚期转录的转换涉及识别新的启动子和抑制在早期多聚腺苷酸化位点的转录终止。我们还确定了一个小外显子与编码潜在的推定E2反式激活蛋白。通过S1定位E2编码外显子和编码高度致瘤CRPV的全尺寸E6蛋白的次要外显子的定量没有显示乳头状瘤和癌之间转录水平的差异。
The capsite of the 2.6- and 4.8-kb major late transcripts of cottontail rabbit papillomavirus (CRPV) has been mapped by primer extension. A leader exon of about 300 nucleotides common to both RNAs is located in the untranslated region of the genome upstream of the capsites for early transcripts. In contrast to the early capsites which are all preceded by TATA boxes, no such sequence is present 30 nucleotides upstream of the late capsite. These data indicate that the switch from early to late transcription involves recognition of a new promoter and suppression of transcription termination at the early polyadenylation site. We have also identified a minor exon with a coding potential for a putative E2 transactivating protein. Quantitation by S1 mapping of the E2 coding exon and a minor exon coding for a full-sized E6 protein unique in size to the highly oncogenic CRPV did not reveal differences in the level of transcription between papillomas and carcinomas.