Dibutyl phthalate-mediated oxidative stress induces splenic injury in mice and the attenuating effects of vitamin E and curcumin
Dibutyl phthalate-mediated oxidative stress induces splenic injury in mice and the attenuating effects of vitamin E and curcumin
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DOI：
10.1016/j.fct.2019.110955
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发表时间：
2020-02-01
影响因子：
4.3
通讯作者：
Yan, Biao
中科院分区：
文献类型：
--
作者：
Wang, Xianliang;Yan, Xu;Yan, Biao
邻苯二甲酸二丁酯 (DBP) 是一种普遍存在的环境污染物，达到一定水平可能对人体健康有害。尽管 DBP 与免疫毒性广泛相关，但 DBP 暴露与脾损伤之间的任何关联仍不清楚。本研究的目的是探讨DBP暴露是否会诱发脾损伤，以及两种抗氧化剂维生素E（VitE）和姜黄素（Cur）对DBP诱发的脾损伤的拮抗作用。测定小鼠脾匀浆中ROS、GSH、T-AOC、IL-1β、TNF-α、细胞色素C、caspase-8、caspase-9和caspase-3的水平。使用H&E和甲苯胺蓝染色观察脾脏中的任何组织病理学变化。采用Janus Green B染色观察线粒体形态。结果表明，暴露于50 mg/kg DBP可引起脾脏组织病理学变化，并导致与氧化应激相关的炎症和细胞凋亡，从而可能导致小鼠脾脏损伤。此外，VitE和Cur均可拮抗DBP诱导的氧化应激，减轻脾损伤。这些发现有助于扩大我们对 DBP 介导的免疫毒性的理解，并表明 VitE 和 Cur 可以减轻 DBP 引起的脾损伤以及可能与 DBP 相关的免疫功能下降。
Dibutyl phthalate (DBP) is a ubiquitous environmental contaminant that at certain levels can be harmful to human health. Although DBP has been widely linked to immunotoxicity, any association between DBP exposure and splenic injury remains unknown. The purpose of this study was to investigate whether DBP exposure can induce splenic injury and the antagonistic effects of two antioxidants, vitamin E (VitE) and curcumin (Cur), on DBP-induced splenic injury. The levels of ROS, GSH, T-AOC, IL-1 beta TNF-alpha, cytochrome C, caspase-8, caspase-9 and caspase-3 in the spleen homogenate of mice were measured. Any histopathological changes in the spleen were observed using H&E and toluidine blue staining. And the morphology of mitochondria was observed using Janus Green B staining. The results indicate that exposure to 50 mg/kg DBP could cause histopathological changes of the spleen and result in inflammation and apoptosis associated with oxidative stress, which may lead to splenic injury in mice. Moreover, both VitE and Cur could antagonize the oxidative stress induced by DBP to reduce splenic injury. These findings help to expand our understanding of DBP-mediated immunotoxicity, and to show that VitE and Cur can alleviate DBP-induced splenic injury and the possible DBP-associated decline in immune function.