Assessment of haemic neoplasia in different soft shell clam Mya arenaria populations from eastern Canada by flow cytometry
Assessment of haemic neoplasia in different soft shell clam Mya arenaria populations from eastern Canada by flow cytometry
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DOI：
10.1016/j.jip.2007.12.005
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发表时间：
2008-06-01
影响因子：
3.4
通讯作者：
Berthe, Franck C. J.
中科院分区：
文献类型：
--
作者：
Delaporte, Maryse;Synard, Stephanie;Berthe, Franck C. J.
摘要沙蚕血液瘤病的诊断通常是通过血液细胞学和组织学来实现的。由于肿瘤细胞显示四倍体DNA含量，血细胞细胞周期分析被开发用作诊断工具。本研究的目的是评估应用流式细胞术程序的细胞周期分析建立的共同的蛤蜊，蛤蜊和评估不同的阈值的四倍体细胞的百分比值，以建立HN疾病状态的个别蛤蜊和蛤蜊种群。从加拿大东部的六个蛤种群的HN状态进行了测定。本研究的结果表明，流式细胞术的程序是有用的，在蛤蜊HN诊断。当出现至少20%的S-4 N期血细胞时，认为个体蛤受到HN影响;当出现少于5%的S-4 N期血细胞时，则为阴性。正如本文所讨论的那样。中间病例代表不确定的诊断，包括难以区分的假阴性或假阳性的蛤蜊。在群体水平上，提出了疾病平均强度的15%的额外阈值，这意味着在群体中有几个个体蛤呈现出超过20%的S-4 N期嗜酸性粒细胞。根据这些阈值，只有一个人口被认为是免费的HN疾病，和一个人口明确受到HN。对于其他四个蛤蜊种群，需要进一步的调查，以发展和使用具体和客观的生物标志物的HN。(c)2007爱思唯尔公司All rights reserved.
Diagnosis of haemic neoplasia (HN) in the soft shell clam, Mya arenaria, is often achieved by hematocytology and histology. Since neoplastic cells display tetraploid DNA contents, haemocyte cell cycle analysis was developed for use as a diagnosis tool. The aim of this study was to assess the application of a flow cytometry procedure of cell cycle analysis established for the common cockle, to clams and to evaluate different thresholds of value for the percentage of tetraploid cells for establishing HN disease status of individual clams and clam populations. HN status of six clam populations from eastern Canada was determined. Results of the present study demonstrate a flow cytometry procedure to be useful for HN diagnosis in clams. Individual clams were considered to be affected by HN when presenting at least 20% of haemocytes in S-4N phase; and negative when presenting less that 5% of haemocytes in S-4N phase. As discussed in this paper.. intermediate cases represent uncertain diagnoses including either false-negative or false-positive clams, which are difficult to discriminate. At a population level, an additional threshold of 15% for the mean intensity of the disease is proposed, which means having in the population several individual clams presenting more than 20% of their haernocytes in S-4N phase. Based on these thresholds of value, only one population was considered as free of HN disease, and one population was unequivocally affected by HN. For the four other clam populations, further investigations are needed toward development and use of specific and objective biomarkers of HN. (c) 2007 Elsevier Inc. All rights reserved.