Proteomic analysis of skeletal muscle in insulin-resistant mice: response to 6-week aerobic exercise.
Proteomic analysis of skeletal muscle in insulin-resistant mice: response to 6-week aerobic exercise.
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DOI：
10.1371/journal.pone.0053887
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Fu L
中科院分区：
文献类型：
--
作者：
Yuan H;Niu Y;Liu X;Yang F;Niu W;Fu L
有氧运动通过一些特定的信号蛋白对体重控制和骨骼肌胰岛素敏感性都有有益的影响。为探讨运动在胰岛素抵抗中的作用靶点，采用蛋白质组学筛选的方法，检测长期有氧运动后胰岛素抵抗小鼠骨骼肌和安静对照小鼠骨骼肌蛋白质表达的差异。18只C57BL/6小鼠随机分为两组：6只给予普通饲料(NC)，12只给予高脂饲料(HFD)喂养10周，建立IR模型。模型组再分为安静对照组(HC，n = 6)和运动组(HE，n = 6)。HE组小鼠进行6周的跑台运动。6周后处死小鼠，解剖骨骼肌。提取总蛋白(n = 6，每组)，进行柠檬酸合成酶、蛋白质组双向图谱分析和免疫印迹分析。NC组和HC组之间有15个蛋白点发生明显变化，HC组和HE组之间有23个蛋白点发生明显变化。这些结果提供了一系列运动训练骨骼肌蛋白质丰度的变化，也为关于运动改善胰岛素抵抗机制的新假说提供了基础。
Aerobic exercise has beneficial effects on both weight control and skeletal muscle insulin sensitivity through a number of specific signaling proteins. To investigate the targets by which exercise exerts its effects on insulin resistance, an approach of proteomic screen was applied to detect the potential different protein expressions from skeletal muscle of insulin-resistant mice after prolonged aerobic exercise training and their sedentary controls. Eighteen C57BL/6 mice were divided into two groups: 6 mice were fed normal chow (NC) and 12 mice were fed high-fat diet (HFD) for 10 weeks to produce an IR model. The model group was then subdivided into HFD sedentary control (HC, n = 6) and HFD exercise groups (HE, n = 6). Mice in HE group underwent 6 weeks of treadmill running. After 6 weeks, mice were sacrificed and skeletal muscle was dissected. Total protein (n = 6, each group) was extracted and followed by citrate synthase, 2D proteome profile analysis and immunoblot. Fifteen protein spots were altered between the NC and HC groups and 23 protein spots were changed between the HC and HE groups significantly. The results provided an array of changes in protein abundance in exercise-trained skeletal muscle and also provided the basis for a new hypothesis regarding the mechanism of exercise ameliorating insulin resistance.