Identification and Characterization of miRNAs and Their Predicted mRNAs in the Larval Development of Pearl Oyster Pinctada fucata
Identification and Characterization of miRNAs and Their Predicted mRNAs in the Larval Development of Pearl Oyster Pinctada fucata
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珍珠贝珠母贝幼虫发育过程中 miRNA 及其预测 mRNA 的鉴定和表征
DOI：
10.1007/s10126-022-10105-3
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发表时间：
2022-03-30
影响因子：
3
通讯作者：
Yu, Dahui
中科院分区：
文献类型：
--
作者：
Chen, Jian;Zhai, Ziqin;Yu, Dahui
作为重要的经济贝类，珍珠贝及其幼虫是研究无脊椎动物幼虫发育分子机制的理想模型。幼体发育直接影响珍珠贝的数量和品质。 MicroRNA (miRNA) 可能在发育中发挥重要作用，但 miRNA 表达对 P. fucata 早期发育的影响仍不清楚。在本研究中，使用 Illumina RNA 测序分析了 7 个不同 P. fucata 发育阶段的 miRNA 和 mRNA 转录组学。在这些阶段共鉴定了329个miRNA，包括87个已知miRNA和242个新miRNA，以及33,550个unigene，包括26,333个已知基因和7217个预测新基因。聚类分析表明，受精卵和承轮细胞之间的 miRNA 数量差异最大。此外，利用整合的mRNA转录组预测相邻发育阶段差异表达miRNA的靶基因，并对靶基因进行基因本体富集分析。使用基因本体注释，鉴定出 100 个不同表达的基因和 95 个差异表达的 miRNA，作为幼虫发育调控的一部分。使用实时 PCR 来鉴定与幼虫发育相关的 8 种 mRNA 和 3 种 miRNA。本研究结果将有助于进一步阐明miRNA在无脊椎动物幼虫发育中的调控机制。
As an important economic shellfish, the pearl oyster, Pinctada fucata, and its larvae are an ideal model for studying molecular mechanisms of larval development in invertebrates. Larval development directly affects the quantity and quality of pearl oysters. MicroRNAs (miRNAs) may play important roles in development, but the effects of miRNA expression on P. fucata early development remain unknown. In this study, miRNA and mRNA transcriptomics of seven different P. fucata developmental stages were analyzed using Illumina RNA sequencing. A total of 329 miRNAs, including 87 known miRNAs and 242 novel miRNAs, and 33,550 unigenes, including 26,333 known genes and 7217 predicted new genes, were identified in these stages. A cluster analysis showed that the difference in the numbers of miRNAs was greatest between fertilized eggs and trochophores. In addition, the integrated mRNA transcriptome was used to predict target genes for differentially expressed miRNAs between adjacent developmental stages, and the target genes were subjected to a gene ontology enrichment analysis. Using the gene ontology annotation, 100 different expressed genes and 95 differentially expressed miRNAs were identified as part of larval development regulation. Real-time PCR was used to identify eight mRNAs and three miRNAs related to larval development. The present findings will be helpful for further clarifying the regulatory mechanisms of miRNA in invertebrate larval development.