The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs
The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs
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DOI：
10.1016/s0015-0282(97)81521-x
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发表时间：
1997-08-01
影响因子：
6.7
通讯作者：
Hiroi, M
中科院分区：
文献类型：
--
作者：
Nakahara, K;Saito, H;Hiroi, M
目的：探讨人卵泡中颗粒膜凋亡小体的发生率与卵泡类固醇浓度的关系。设计：132个个体卵泡的病例对照前瞻性研究。地点：手术在山形大学医院进行。患者：36例输卵管性不孕的正常排卵妇女采用常规超刺激法进行IVF-ET诱导排卵。干预措施：患者在给予hCG后进行卵泡抽吸。主要观察指标：荧光显微镜观察恢复颗粒细胞的细胞核，并统计凋亡小体的发生率。滤泡内类固醇主要通过RIA评估。对这些数据进行卵母细胞回收、卵母细胞成熟度、受精和胚胎质量的分析。结果：卵母细胞随后受精的卵泡中的颗粒膜细胞凋亡小体的发生率明显低于卵母细胞未受精的卵泡。卵母细胞发育质量较好的卵泡中膜粒细胞的凋亡小体发生率明显低于卵母细胞发育质量一般和较差的卵泡。在大多数情况下，壁粒细胞区凋亡小体的发生率明显高于积云细胞区。卵母细胞组间卵泡内E-2、P和游离T水平无显著差异。结论：这些结果表明单个卵泡中凋亡小体的发生率较低与卵母细胞的预后较好相关。此外，在卵泡成熟过程中，每个卵泡内的壁粒细胞和积云细胞也可能出现分化。(C) 1997年，美国生殖医学学会。
Objective: To investigate the relationship between the incidence of apoptotic bodies in membrana granulosa and follicular steroid concentrations in human follicles.Design: Case-controlled prospective study for 132 individual follicles.Setting: Procedures were performed in Yamagata University Hospital.Patient(s): Thirty-six normo-ovulatory women with tubal infertility underwent ovulation induction for IVF-ET with a conventional hyperstimulation method.Intervention(s): Patients underwent follicle aspiration after the administration of hCG.Main Outcome Measure(s): The nuclei of recovered granulosa cells were examined by fluorescence microscopy, and the incidence of apoptotic bodies was tabulated. Intrafollicular steroids were evaluated mainly by RIA. These data were analyzed with respect to oocyte-retrieval, oocyte maturity, fertilization, and embryo quality.Result(s): Membrana granulosa cells in the follicles from which oocytes were subsequently fertilized showed a significantly lower incidence of apoptotic bodies than those in follicles from which the oocytes did not fertilize. Membrana granulosa cells in the follicles from which oocytes were developed into good quality showed a significantly lower incidence of apoptotic bodies than those in the follicles from which oocytes developed into fair and poor quality. The incidence of apoptotic bodies was significantly higher in the mural granulosa cell region than in the cumulus cell region in most cases. Intrafollicular E-2, P, and free T levels were not different between the oocyte groups.Conclusion(s): These results indicate that lower incidence of apoptotic bodies in individual follicles is associated with better outcomes for oocytes. Also, mural granulosa cells and cumulus cells in each follicle may show differentiation during follicular maturation. (C) 1997 by American Society for Reproductive Medicine.