Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue
Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue
复制标题
硫酸盐还原菌与生物浸出嗜酸菌竞争生长，用于堆生物浸出残留物的生物修复
DOI：
10.3390/ijerph17082715
复制
发表时间：
2020-04-01
影响因子：
--
通讯作者：
Ruan, Renman
中科院分区：
文献类型：
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作者：
Phyo, Aung Kyaw;Jia, Yan;Ruan, Renman
含硫化物矿物的采矿废石天然地为铁和硫氧化微生物提供了栖息地，它们通过促进硫化物矿物的氧化而加速酸性矿山废水（AMD）的产生。硫酸盐还原菌（SRB）有时被用于通过微生物诱导的金属硫化物沉淀来处理AMD溶液。首次尝试在黄铁矿堆生物浸出残渣中直接生长硫酸盐还原菌，以与当地的铁和硫氧化微生物竞争。酸性硫酸盐还原菌和铁还原菌在pH 2.0和3.0下培养。将其应用于酸性堆浸渣中，结果表明，pH升高和有机质是它们与当地嗜酸菌竞争的重要因素。添加有机质促进了SRB和铁还原菌的生长，抑制了铁硫氧化菌的生长，尤其是有机质与石灰的共同作用。在硫酸盐还原菌和铁还原微生物的作用下，pH由酸性上升到近中性，Eh下降，金属与微生物生成的硫化物一起沉淀，导致残渣孔隙溶液中Cu含量很低。结果表明，酸性矿山废水直接在黄铁矿废石中原位抑制，促进SRB和铁还原微生物的生长，与当地的铁和硫氧化微生物竞争，可用于硫化物废石中AMD的源头控制和最终修复。
Abstracts Mining waste rocks containing sulfide minerals naturally provide the habitat for iron- and sulfur-oxidizing microbes, and they accelerate the generation of acid mine drainage (AMD) by promoting the oxidation of sulfide minerals. Sulfate-reducing bacteria (SRB) are sometimes employed to treat the AMD solution by microbial-induced metal sulfide precipitation. It was attempted for the first time to grow SRB directly in the pyritic heap bioleaching residue to compete with the local iron- and sulfur-oxidizing microbes. The acidic SRB and iron-reducing microbes were cultured at pH 2.0 and 3.0. After it was applied to the acidic heap bioleaching residue, it showed that the elevated pH and the organic matter was important for them to compete with the local bioleaching acidophiles. The incubation with the addition of organic matter promoted the growth of SRB and iron-reducing microbes to inhibit the iron- and sulfur-oxidizing microbes, especially organic matter together with some lime. Under the growth of the SRB and iron-reducing microbes, pH increased from acidic to nearly neutral, the Eh also decreased, and the metal, precipitated together with the microbial-generated sulfide, resulted in very low Cu in the residue pore solution. These results prove the inhibition of acid mine drainage directly in situ of the pyritic waste rocks by the promotion of the growth of SRB and iron-reducing microbes to compete with local iron and sulfur-oxidizing microbes, which can be used for the source control of AMD from the sulfidic waste rocks and the final remediation.