Mouse sperm lacking ADAM1b/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization
Mouse sperm lacking ADAM1b/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization
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DOI：
10.1074/jbc.m510558200
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发表时间：
2006-03-03
影响因子：
4.8
通讯作者：
Baba, T
中科院分区：
文献类型：
--
作者：
Kim, E;Yamashita, M;Baba, T
受精素是一种由精子表面的α(ADAM1)和β(ADAM2)亚基组成的异源二聚体蛋白质复合体，被认为介导了精子和卵子质膜之间的黏附和融合。在这里，我们已经证明了缺乏ADAM1b的突变雄性小鼠是可育的，并且ADAM1b的丢失不会导致精子功能的显著缺陷，如从子宫迁移到输卵管，与卵子透明带结合，以及与无透明带卵子融合。ADAM1b缺陷的附睾精子显示细胞表面的ADAM2严重减少，尽管睾丸生殖细胞中存在正常的ADAM2。ADAM1b和ADAM2在精子表面的出现取决于睾丸生殖细胞中ADAM1b/ADAM2受精素的形成和丰度。这些结果表明，小鼠ADAM1b/ADAM2受精素可能不是在精子/卵子的融合中起关键作用，而是在精子表面出现这两个ADAM中起关键作用。
Fertilin, a heterodimeric protein complex composed of alpha (ADAM1) and beta (ADAM2) subunits on the sperm surface, is believed to mediate adhesion and fusion between the sperm and egg plasma membranes. Here we have shown that mutant male mice lacking ADAM1b are fertile and that the loss of ADAM1b results in no significant defect in sperm functions such as migration from the uterus into oviduct, binding to egg zona pellucida, and fusion with zona pellucida-free eggs. ADAM1b-deficient epididymal sperm showed a severe reduction of ADAM2 on the cell surface, despite the normal presence of ADAM2 in testicular germ cells. The appearance of ADAM1b and ADAM2 on the sperm surface depended on formation and abundance of ADAM1b/ADAM2 fertilin in testicular germ cells. These results suggest that mouse ADAM1b/ADAM2 fertilin may play a crucial role not in the sperm/ egg fusion but in the appearance of these two ADAMs on the sperm surface.