Enhanced bone regeneration and visual monitoring via superparamagnetic iron oxide nanoparticle scaffold in rats
Enhanced bone regeneration and visual monitoring via superparamagnetic iron oxide nanoparticle scaffold in rats
复制标题
通过超顺磁性氧化铁纳米颗粒支架增强大鼠骨再生和视觉监测
DOI：
10.1002/term.2641
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发表时间：
2018-04-01
影响因子：
3.3
通讯作者：
Xu, Hockin H. K.
中科院分区：
文献类型：
--
作者：
Hu, Shuying;Zhou, Yi;Xu, Hockin H. K.
在骨工程中使用支架的主要挑战涉及体内非侵入性监测和增强骨再生。超顺磁性氧化铁纳米颗粒（SPION）的组织修复作用已被本研究组证实。然而，需要体内测试来确认体外结果。在此，将装载SPION的明胶海绵（GS）用作支架（SPIONs-GS）并植入Sprague-Dawley大鼠的切牙窝中。空白和GS填充的切牙窝作为对照。分别于第2周和第4周处死大鼠。SPIONs-GS组T2加权磁共振成像（MRI）信号强度显著降低。支架的图像强度的变化（指示支架降解和与宿主组织的相互作用）可以随时间视觉监测。显微计算机断层扫描显示，SPIONs-GS组在4周时比空白对照组有更多的新骨形成（64.44 +/- 10.92 vs. 28.1 +/- 4.49，p < .0001）和更好的牙槽嵴保存（0.962 +/- 0.01 vs. 0.92 +/-0.01，p < .0001）。组织学证实了成像结果，显示新骨形成和支架降解具有良好的一致性。由于GS的快速降解，SPION的数量随着时间的推移而迅速减少，而细胞内吞SPION的数量随着时间的推移而增加。4周时MRI可检测到残留SPION和新骨形成。因此，很明显SPION诱导了活跃的骨生成。总之，在不使用外部磁场的情况下，通过在体内植入SPIONs-GS可以获得良好的MRI可见性和增强的骨再生。
A main challenge for use of scaffolds in bone engineering involves non-invasive monitoring in vivo and enhanced bone regeneration. The tissue repair effect of superparamagnetic iron oxide nanoparticles (SPIONs) was demonstrated previously by our group. However, testing in vivo is needed to confirm in vitro results. Here, SPIONs loaded gelatin sponge (GS) was used as a scaffold (SPIONs-GS) and implanted in the incisor sockets of Sprague-Dawley rats. Incisor sockets filled with nothing and filled with GS served as controls. Rats were sacrificed at 2 and 4weeks. A significant decrease in the signal intensity of T2-weighted magnetic resonance imaging (MRI) in the SPIONs-GS group was noted. Changes in image intensity of scaffolds (indicating scaffold degradation and interaction with host tissues) could be visually monitored over time. Microcomputed tomography showed that the SPIONs-GS group had more newly formed bone (64.44 +/- 10.92 vs. 28.1 +/- 4.49, p < .0001) and a better preserved alveolar ridge than blank control group at 4 weeks (0.962 +/- 0.01 vs. 0.92 +/- 0.01, p < .0001). Histology confirmed imaging results, showing good consistency in new bone formation and scaffold degradation. The number of SPIONs decreased rapidly with time due to quick degradation of GS, whereas the number of endocytic SPIONs in cells increased with time. These residual SPIONs, together with newly formed bone, could be detected by MRI at 4weeks. Therefore, it was clear that SPIONs induced active osteogenesis. In conclusion, good visibility on MRI and enhanced regeneration of bone can be obtained by implanting SPIONs-GS in vivo without using an external magnetic field.