The 28-kDa calbindin-D is a major calcium-binding protein in the basilar papilla of the chick.
The 28-kDa calbindin-D is a major calcium-binding protein in the basilar papilla of the chick.
复制标题
28 kDa 钙结合蛋白-D 是雏鸡基底乳头中的主要钙结合蛋白。
DOI：
10.1073/pnas.85.10.3387
复制
发表时间：
1988
影响因子：
11.1
通讯作者：
Matschinsky,FM
中科院分区：
文献类型：
--
作者：
Oberholtzer,JC;Buettger,C;Summers,MC;Matschinsky,FM
在以前的工作中，我们发现了一种基底乳头蛋白(BPP23)，它似乎是鸡耳蜗基乳头中含量最丰富的可溶性蛋白质之一。本文报道了鸡耳蜗蛋白BPP23的纯化和特异性抗血清的制备。免疫印迹和免疫沉淀实验表明，BPP23是一种钙结合蛋白，即使不完全相同，也与禽类钙结合蛋白非常相似，后者是一种28 kDa的维生素D依赖的钙结合蛋白。虽然基底乳头同时含有受体毛细胞和支持细胞，但其他人的免疫细胞化学研究已定位于大鼠听觉感受器上皮内毛细胞的钙结合素样免疫反应。我们对BPP23蛋白丰度的估计，假设只在毛细胞内定位，表明浓度至少为1 mM。禽类钙结合蛋白有4个高亲和力的钙结合位点(Kd=0.5×10~(-6))。如此高水平的钙结合活性提示耳蜗钙结合蛋白(BPP23)在毛细胞钙稳态和听觉传导中具有重要作用。
In previous work we identified a basilar papilla protein (BPP23) that appears to be one of the most abundant soluble proteins in the basilar papilla of the chick cochlea. Here we report the purification of protein BPP23 from chick cochlea and the generation of a specific antiserum. Immunoblotting and immunoprecipitation experiments with this antiserum indicate that BPP23 is a calcium-binding protein very similar, if not identical, to avian calbindin, the 28-kDa vitamin D-dependent calcium-binding protein. Although the basilar papilla contains both receptor hair cells and supporting cells, immunocytochemical studies by others have localized calbindin-like immunoreactivity to the hair cells in the rat auditory receptor epithelium. Our estimates of the abundance of protein BPP23, assuming exclusive localization within the hair cell, indicate a concentration of at least 1 mM. Avian calbindin has four high-affinity (Kd = 0.5 X 10(-6)) calcium-binding sites. The presence of a specific calcium-binding activity at such high levels suggests an important function for cochlear calbindin (BPP23) in hair cell calcium homeostasis and auditory transduction.
听力神经生物学：耳蜗
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
R. Altschuler;R. Bobbin;D. Hoffman
通讯作者：
D. Hoffman