Response of genes involved in lipid metabolism in rat epididymal white adipose tissue to different fasting conditions after long-term fructose consumption
Response of genes involved in lipid metabolism in rat epididymal white adipose tissue to different fasting conditions after long-term fructose consumption
复制标题
长期果糖摄入后大鼠附睾白色脂肪组织脂质代谢相关基因对不同禁食条件的响应
DOI：
10.1016/j.bbrc.2017.01.119
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发表时间：
2017-03-04
影响因子：
3.1
通讯作者：
Wang, Jian-wei
中科院分区：
文献类型：
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作者：
Li, Jin-xiu;Ke, Da-zhi;Wang, Jian-wei
饮食中的果糖会导致代谢综合征的发生，这一点一直备受关注。高果糖饮食改变参与脂质代谢的基因表达。在禁食再喂养的模型大鼠/小鼠中，高碳水化合物饮食增加了许多肝脂肪生成酶的水平。白色脂肪组织（WAT）和肝脏在维持营养平衡中起着关键作用。本研究的目的是分析长期慢性果糖摄入后，不同禁食条件下附睾WAT（eWAT）中脂质代谢相关关键基因的表达。给大鼠喂食补充有10%w/v果糖溶液的标准食物5周，并在禁食食物和果糖戒断（禁食）或禁食食物和持续果糖（仅果糖）14 h后处死。分析eWAT的血液参数和参与脂肪酸合成（ChREBP、SREBP-1c、FAS、SCD 1）、甘油三酯生物合成（DGAT-1、DGAT-2）和脂质动员（ATGL、HSL）的基因的表达。此外，还检测了PPAR-gamma、CD 36和LPL的mRNA水平。正如预期的那样，单独的果糖增加FAS，SCD 1的mRNA表达，并相应地降低ATGL和HSL mRNA水平。禁食状态下，ChREBP、DGAT-2、ATGL和HSL mRNA水平接近正常，FAS和SCD 1 mRNA水平接近基础水平。果糖组SREBP-1c、PPAR-gamma和LPL mRNA表达无明显变化，而CD 36 mRNA表达明显降低。总之，这些研究结果表明，参与脂质代谢的基因在大鼠eWAT是不同的，在不同的禁食条件下，长期果糖消费后。(C)2017由Elsevier Inc.出版
There has been much concern regarding the dietary fructose contributes to the development of metabolic syndrome. High-fructose diet changes the expression of genes involved in lipid metabolism. Levels of a number of hepatic lipogenic enzymes are increased by a high-carbohydrate diet in fasted-refed model rats/mice. Both the white adipose tissue (WAT) and the liver play a key role in the maintenance of nutrient homeostasis. Here, the aim of this study was to analyze the expression of key genes related to lipid metabolism in epididymal WAT (eWAT) in response to different fasting condition after long-term chronic fructose consumption. Rats were fed standard chow supplemented with 10% w/v fructose solution for 5 weeks, and killed after chow-fasting and fructose withdrawal (fasting) or chow fasting and continued fructose (fructose alone) for 14 h. Blood parameters and the expression of genes involved in fatty acid synthesis (ChREBP, SREBP-1c, FAS, SCD1), triglyceride biosynthesis (DGAT-1, DGAT-2) and lipid mobilization (ATGL, HSL) in eWAT were analyzed. In addition, mRNA levels of PPAR-gamma, CD36 and LPL were also detected. As expected, fructose alone increased the mRNA expression of FAS, SCD1, and correspondingly decreased ATGL and HSL mRNA levels. However, ChREBP, DGAT-2, ATGL and HSL mRNA levels restored near to normal while FAS and SCD1 tend to basic level under fasting condition. The mRNA expression of SREBP-1c, PPAR-gamma and LPL did not changed at any situations but CD36 mRNA decreased remarkably in fructose alone group. In conclusion, these findings demonstrate that genes involved in lipid metabolism in rat eWAT are varied in response to different fasting conditions after long-term fructose consumption. (C) 2017 Published by Elsevier Inc.