ZEISS Airyscan: Optimizing Usage for Fast, Gentle, Super-Resolution Imaging.
ZEISS Airyscan: Optimizing Usage for Fast, Gentle, Super-Resolution Imaging.
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DOI：
10.1007/978-1-0716-1402-0_5
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Hammer JA
中科院分区：
文献类型：
--
作者：
Wu X;Hammer JA
蔡司Airyscan显微镜使用专门的32通道Airyscan探测器将衍射限制的点扫描共聚焦显微镜转变为超分辨率显微镜。通过提高分辨率2倍和信噪比8倍相对于传统的共聚焦显微镜，同时保留共聚焦功能，Airyscan显微镜已成为细胞生物学家非常流行的超分辨率成像工具。在本章中，我们将介绍Airyscan成像的基本原理，旨在帮助读者为不同类型的实验确定适当的采集设置，优化成像条件，并处理原始Airyscan图像以获得最佳质量的最终图像。我们还提供了一些Airyscan成像的提示、技巧和最佳实践。值得注意的是，虽然我们的重点是这台显微镜的Airyscan功能，而不是其传统的共聚焦功能，Airyscan单元来作为一个附加到传统的蔡司激光扫描共聚焦显微镜。本方案适用于第一代Airyscan Zeiss 800系列显微镜。
The Zeiss Airyscan microscope transforms a diffraction-limited, point-scanning confocal microscope into a super-resolution microscope using a specialized 32-channel Airyscan detector. By improving resolution two-fold and signal-to-noise ratio eight-fold relative to conventional confocal microscopes while retaining confocal functionality, the Airyscan microscope has become a very popular super-resolution imaging tool for cell biologists. In this chapter, we describe the fundamentals of Airyscan imaging, with the aim of helping the reader determine the proper acquisition settings for different types of experiments, optimize imaging conditions, and process the raw Airyscan images to obtain final images with the best quality. We also provide some tips, tricks, and best practices for Airyscan imaging. Of note, while our focus is on the Airyscan function of this microscope rather than its conventional confocal function, the Airyscan unit comes as an add-on to the conventional Zeiss laser scanning confocal microscope. This protocol is for the first generation Airyscan Zeiss 800 series microscope.