Luteinizing hormone modulates intracellular calcium, protein tyrosine phosphorylation and motility during human sperm capacitation
Luteinizing hormone modulates intracellular calcium, protein tyrosine phosphorylation and motility during human sperm capacitation
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DOI：
10.1016/j.bbrc.2017.01.010
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发表时间：
2017-02-05
影响因子：
3.1
通讯作者：
Chirinos, Mayel
中科院分区：
文献类型：
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作者：
Lopez-Torres, Aidee S.;Gonzalez-Gonzalez, Maria E.;Chirinos, Mayel
为了受精，精子在到达并与卵母细胞融合之前，必须经历生理和生化变化，这一过程被称为获能。与获能相关的精子修饰是通过与女性生殖道中存在的分子相互作用来调节的。在女性的生育窗口期，一些生殖激素在血清中的浓度达到最大值，如黄体生成素（LH）。由于准备受精的精子可能暴露于LH，本研究的目的是研究这种激素对细胞内Ca2+浓度（[Ca2+];）、蛋白酪氨酸磷酸化、精子活力和顶体反应在获能条件下的影响。结果表明，LH增加Ca2+振荡的持续时间和振幅。此外，运动分析表明，LH降低了快速进行性运动，精子的过度激活以及一些动力学参数在激素0.5和1的存在下增加。此外，这两种激素浓度也持续促进蛋白酪氨酸磷酸化。但未观察到对顶体反应的影响。总之，有证据表明黄体生成素调节了几个与获能有关的精子功能变量，这表明黄体生成素在人类受精过程中可能具有重要而未被探索的作用。(C) 2017爱思唯尔公司版权所有。
In order to fertilize, spermatozoa must undergo physiological and biochemical changes during their transit along the female reproductive tract before reaching and fusing with the oocyte, process known as capacitation. Sperm modifications associated with capacitation are modulated by their interaction with molecules present in the female reproductive tract. During the woman fertile window, some reproductive hormones reach their maximum concentrations in serum, such as the luteinizing hormone (LH). Since spermatozoa preparing to fertilize may be exposed to LH, the purpose of this work was to study the effects of this hormone on intracellular Ca2+ concentrations ([Ca2+];), protein tyrosine phosphorylation, sperm motility and acrosome reaction under capacitating conditions. The results showed that LH increases the duration and amplitude of Ca2+ oscillations. Furthermore, motility analysis indicated that LH decreases rapid progressive motility and that sperm hyperactivation as well as several kinetic parameters augment in the presence of 0.5 and 1 of the hormone. In addition, these two hormone concentrations also consistently promoted protein tyrosine phosphorylation. However, no effects on acrosome reaction were observed. In conclusion, the evidence indicates that LH modulates several sperm function variables involved in capacitation, suggesting that may have an important and unexplored role during human fertilization. (C) 2017 Elsevier Inc. All rights reserved.