Farnesol, a common sesquiterpene, inhibits PQS production in Pseudomonas aeruginosa
Farnesol, a common sesquiterpene, inhibits PQS production in Pseudomonas aeruginosa
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DOI：
10.1111/j.1365-2958.2007.05840.x
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发表时间：
2007-08-01
影响因子：
3.6
通讯作者：
Hogan, Deborah A.
中科院分区：
文献类型：
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作者：
Cugini, Carla;Calfee, M. Worth;Hogan, Deborah A.
法尼醇是一种倍半萜，由许多生物产生，包括白色念珠菌。在这里，我们报道了向铜绿假单胞菌（一种机会性人类细菌病原体）的培养中添加法尼醇，导致喹诺酮假单胞菌信号（PQS）和poss控制的毒力因子pyocyanin的产生减少。添加法尼醇15分钟内，观察到PQS生物合成操纵子的第一个基因pqsA的转录水平下降。编码pqsA正调控转录因子的pqsR （mvfR）的转录水平未受影响。一株产生PqsR并含有pqsA启动子与lacZ融合的大肠杆菌菌株同样表明，法尼醇抑制了pqs刺激的转录。电泳迁移率转移实验显示，与PQS一样，法尼醇刺激PqsR在先前表征的LysR结合位点与pqsA启动子结合，表明法尼醇促进了PqsR与pqsA启动子之间的非有效相互作用。与白色念珠菌一起生长导致铜绿假单胞菌PQS和pyocyanin的产生减少，这表明真菌产生的法尼醇的数量足以影响铜绿假单胞菌PQS信号传导。相关的类异戊二烯化合物，而不是其他长链醇，也抑制了微摩尔浓度下PQS的产生，这表明相关化合物可能参与了铜绿假单胞菌的种间相互作用。
Farnesol is a sesquiterpene produced by many organisms, including the fungus Candida albicans. Here, we report that the addition of farnesol to cultures of Pseudomonas aeruginosa, an opportunistic human bacterial pathogen, leads to decreased production of the Pseudomonas quinolone signal (PQS) and the POS-controlled virulence factor, pyocyanin. Within 15 min of farnesol addition, decreased transcript levels of pqsA, the first gene in the PQS biosynthetic operon, were observed. Transcript levels of pqsR (mvfR), which encodes the transcription factor that positively regulates pqsA, were unaffected. An Escherichia coli strain producing PqsR and containing the pqsA promoter fused to lacZ similarly showed that farnesol inhibited PQS-stimulated transcription. Electrophoretic mobility shift assays showed that, like PQS, farnesol stimulated PqsR binding to the pqsA promoter at a previously characterized LysR binding site, suggesting that farnesol promoted a non-productive interaction between PqsR and the pqsA promoter. Growth with C. albicans leads to decreased production of PQS and pyocyanin by P. aeruginosa, suggesting that the amount of farnesol produced by the fungus is sufficient to impact P aeruginosa PQS signalling. Related isoprenoid compounds, but not other long-chain alcohols, also inhibited PQS production at micromolar concentrations, suggesting that related compounds may participate in interspecies interactions with P. aeruginosa.