The Arabidopsis thaliana DSB formation (AtDFO) gene is required for meiotic double-strand break formation.
The Arabidopsis thaliana DSB formation (AtDFO) gene is required for meiotic double-strand break formation.
复制标题
DOI：
10.1111/j.1365-313x.2012.05075.x
复制
发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Cheng Zhang;Yao Song;Zhihao Cheng;Yingxiang Wang;Jun Zhu;Hong Ma;Ling Xu;Zhongnan Yang
中科院分区：
文献类型：
--
作者：
Cheng Zhang;Yao Song;Zhihao Cheng;Yingxiang Wang;Jun Zhu;Hong Ma;Ling Xu;Zhongnan Yang
DNA双链断裂（DSB）的形成是由保守的Spo 11蛋白催化的减数分裂重组的起始事件。在拟南芥中，已经报道了几种蛋白质参与DSB的形成。在这里，我们报告了一个拟南芥DSB形成（DFO）基因在拟南芥中，参与DSB的形成。与野生型的四分体不同，dfo突变体表现出育性降低，产生的多分体具有异常数量的小孢子。dfo性母细胞同源染色体联会和分离缺陷。遗传分析表明，Atdfo-1的同源重组在减数分裂前期I受到严重影响。DFO编码的蛋白质没有任何已知的保守结构域。在植物界之外没有发现同源物，表明AtDFO是一种植物特异性蛋白。据报告，AtMRE 11负责处理SPO 11生成的DSB。Atmre 11突变体在减数分裂期间显示染色体断裂。然而，Atdfo Atmre 11双突变体没有这样的染色体断裂，表明AtDFO是DSB形成所必需的。
DNA double-strand break (DSB) formation is the initial event for meiotic recombination catalyzed by the conserved Spo11 protein. In Arabidopsis, several proteins have been reported to be involved in DSB formation. Here, we report an Arabidopsis DSB forming (DFO) gene in Arabidopsis that is involved in DSB formation. The dfo mutant exhibits reduced fertility, producing polyads with an abnormal number of microspores, unlike the tetrads in the wild type. The dfo meiocytes were defective in homologous chromosome synapsis and segregation. Genetic analysis revealed that the homologous recombination of Atdfo-1 is severely affected in meiotic prophase I. DFO encodes a protein without any known conserved domain. There was no homologue identified outside the plant kingdom, indicating that AtDFO is a plant-specific protein. AtMRE11 has been reported to be responsible for processing SPO11-generated DSBs. The Atmre11 mutant displays chromosome fragmentation during meiosis. However, the Atdfo Atmre11 double mutant had no such chromosome fragmentation, indicating that AtDFO is required for DSB formation.