CD133+ single cell-derived progenies of colorectal cancer cell line SW480 with different invasive and metastatic potential
CD133+ single cell-derived progenies of colorectal cancer cell line SW480 with different invasive and metastatic potential
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具有不同侵袭和转移潜力的结直肠癌细胞系 SW480 的 CD133 单细胞衍生后代
DOI：
10.1007/s10585-010-9341-0
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发表时间：
2010-10-01
影响因子：
4
通讯作者：
Ding, Yanqing
中科院分区：
文献类型：
--
作者：
Li, Guangqiu;Liu, Chao;Ding, Yanqing
单细胞子代（SCPs）从其等基因母细胞继承生物学特性。如果单个癌细胞能够产生后代，并能在体外持续传代，并在异种移植中产生肿瘤，那么该细胞应该是癌起始细胞。CD133 （pronin -1, Prom1）是人类结直肠癌（CRC）干细胞的标志物，也可能是转移性癌症干细胞（CSCs）的标志物。采用有限稀释法分离CRC细胞系SW480 33个SCPs，其中CD133阳性30个，阴性3个。所有CD133(+) SCPs都具有致瘤性，皮下肿瘤迅速扩大，而3个CD133(-) SCPs中只有1个在裸鼠中发展成最小的肿瘤。原位移植实验表明，CD133(+) SCPs在肠壁侵袭、淋巴结和肝脏转移方面具有异质性。CD133(+) SCPs在细胞生长、侵袭能力、上皮-间质转化和与转移潜能相关的CSCs标志物（CD133、CD44和CXCR4）的表达方面存在差异。CD133(-) SCPs不产生继发性移植瘤、肠道侵袭和转移。结果表明SW480 SCPs的CD133（+）亚群具有异质性的侵袭和转移能力，CRC-CSCs可能是一个异质性亚群。
Single cell progenies (SCPs) inherit biological properties from their isogenetic mother cells. If a single cancer cell can give rise to progenies, which can be passaged sustainably in vitro and produce tumor in xenotransplantation, the cell should be cancer initiating cell. CD133 (Prominin-1, Prom1) is the marker of human colorectal cancer (CRC) stem cells and probably a marker of metastatic cancer stem cells (CSCs). Thirty-three SCPs of CRC cell line SW480 were isolated by limited dilution methods, thirty of which are CD133 positive and three negative. All of the CD133(+) SCPs are tumorigenic, and the subcutaneous tumors expanded rapidly, while only 1 of 3 CD133(-) SCPs developed a minimal tumor in nude mice. Orthotopic transplantation experiments showed that CD133(+) SCPs possessed heterogeneity in intestinal wall invasion, lymph node and liver metastases. CD133(+) SCPs varied in cell growth, invasive ability, epithelial-mesenchymal-transition and expression of CSCs markers (CD133, CD44, and CXCR4) associated with metastatic potential. CD133(-) SCPs did not produce secondary transplanted tumor, intestinal invasion and metastasis. The results indicated CD133(+) subpopulation of SW480 SCPs bear heterogeneous invasive and metastatic ability, and CRC-CSCs might be a heterogeous subpopulation.