WOX gene phylogeny in Poaceae:: A comparative approach addressing leaf and embryo development
WOX gene phylogeny in Poaceae:: A comparative approach addressing leaf and embryo development
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DOI：
10.1093/molbev/msm182
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发表时间：
2007-11-01
影响因子：
10.7
通讯作者：
Werr, Wolfgang
中科院分区：
文献类型：
--
作者：
Nardmann, Judith;Zimmermann, Roman;Werr, Wolfgang
基于WOX（WUSCHEL相关同源框基因家族）的同源域（HD）氨基酸序列，在禾本科的3个主要辐射族：早熟禾亚科（Brachypodium distachyon）、竹亚科（Bambusoideae）和黍亚科（Panicoideae）（Zea mays）中建立了同源性。所有3种禾本科植物的基因组在WOX 3分支中都含有一个古老的重复，玉米和水稻的细胞表达模式表明在叶片发育过程中旁系同源物的亚功能化，这可能与禾本科植物叶片的结构和茎的环绕有关。玉米WOX基因家族成员作为分子标记在玉米胚胎发育中的首次使用使我们能够可视化玉米原胚中的细胞决定，包括与合子的顶基极性成斜角的芽/根轴的规格。所有的分子标记数据是兼容的结论，胚胎芽/根轴包括一个离散的域从早期原胚阶段向前。新的细胞命运的芽和根内获得这个独特的形态发生轴域，延长，从而分离芽顶端分生组织和根顶端分生组织（RAM）在玉米胚原基。
The phylogeny based on the homeodomain (HD) amino acid sequence of the WOX (WUSCHEL-related homeobox gene family) was established in the 3 major radiations of the Poaceae family: Pooideae (Brachypodium distachyon), Bambusoideae (Oryza sativa), and Panicoideae (Zea mays). The genomes of all 3 grasses contain an ancient duplication in the WOX3 branch, and the cellular expression patterns in maize and rice indicate subfunctionalization of paralogues during leaf development, which may relate to the architecture of the grass leaf and the encircling of the stem. The use of maize WOX gene family members as molecular markers in maize embryo development for the first time allowed us to visualize cellular decisions in the maize proembryo, including specification of the shoot/root axis at an oblique angle to the apical-basal polarity of the zygote. All molecular marker data are compatible with the conclusion that the embryonic shoot/root axis comprises a discrete domain from early proembryo stages onward. Novel cell fates of the shoot and the root are acquired within this distinct morphogenic axis domain, which elongates and thus separates the shoot apical meristem and root apical meristem (RAM) anlagen in the maize embryo.