Insertion of a transposable element in Less Shattering1 (SvLes1) gene is not always involved in foxtail millet (Setaria italica) domestication
Insertion of a transposable element in Less Shattering1 (SvLes1) gene is not always involved in foxtail millet (Setaria italica) domestication
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DOI：
10.1007/s10722-021-01165-w
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发表时间：
2021-03-15
影响因子：
2
通讯作者：
Ito, Kazue
中科院分区：
文献类型：
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作者：
Fukunaga, Kenji;Matsuyama, Sarasa;Ito, Kazue
近年来，在狗尾草中发现了一个位于V染色体上的MYB转录因子，即小破碎1基因（SvLes1），该基因控制狗尾草种子破碎的程度。绿色的SvLes1 - 1和SvLes1 - 2分别是野生型（高落粒率）等位基因和低落粒率等位基因。除了这两个等位基因外，在谷子中还发现了一个通过在外显子2插入转座元件（TE）而丧失功能的等位基因。viridis，命名为SiLes1-TE。该基因被认为是谷子中的驯化基因。我们筛选了131个谷子品种，发现16个谷子地方品种（12.2%）不具有TE，尽管表达了非落粒表型。我们对这16份材料的SvLes1基因进行了测序，并将它们分为三个等位基因SvLes1 - 1，SvLes1 - 2和一个新的等位基因SvLes1 - 3。这三个等位基因的地理分布是不同的，这表明谷子驯化和分化比预期的要复杂。
Recently, the less Shattering1 gene (SvLes1), an MYB transcription factor on chromosome V, in Setaria viridis, was reported to control the degree of seed shattering within S. viridis. SvLes1-1 and SvLes1-2 are the wild type (high shattering) allele and the reduced shattering allele, respectively. In addition to these two alleles, the loss-of-function allele through a transposable-element (TE) insertion in exon 2 was found in foxtail millet, a domesticated type of S. viridis, and was designated as SiLes1-TE. This gene is considered to be a domestication gene in foxtail millet. We screened 131 accessions of foxtail millet and found that 16 landraces (12.2%) of foxtail millet do not have the TE, despite expression of the non-shattering phenotype. We sequenced the SvLes1 gene of these 16 accessions and classified them into three alleles, SvLes1-1, SvLes1-2, and a new allele, SvLes1-3. The geographical distribution of these three alleles was different, suggesting that foxtail millet domestication and differentiation are more complex than expected.