Microorganisms Identified in the Maternal Bladder: Discovery of the Maternal Bladder Microbiota.
Microorganisms Identified in the Maternal Bladder: Discovery of the Maternal Bladder Microbiota.
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DOI：
10.1055/s-0037-1606860
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发表时间：
2017-07
期刊：
影响因子：
0.9
通讯作者：
Waters TP
中科院分区：
文献类型：
--
作者：
Jacobs KM;Thomas-White KJ;Hilt EE;Wolfe AJ;Waters TP
目的探讨妊娠期膀胱微生物群的特征。方法对51例在三级医院接受直置导尿或经尿道Foley导尿的孕妇进行前瞻性观察研究。16S rRNA基因测序和增强定量尿液培养评估母体膀胱微生物群，并与标准尿液培养结果进行比较。结果加强尿液培养和16S rRNA基因测序，在大多数参与者中检测到细菌。乳酸菌和加德纳菌是最常见的微生物。相比之下，标准尿液培养有100%的假阴性率，并且未能检测到几种已知或新出现的泌尿病原体。结论大多数孕妇膀胱中存在活菌。这对无症状细菌尿的定义提出了挑战。
Objective The objective of this study was to characterize the bladder microbiota in pregnancy. Methods A prospective observational study of 51 pregnant women, admitted to a tertiary care hospital, who underwent straight catheterization urine collection or transurethral Foley catheter placement. 16S rRNA gene sequencing and enhanced quantitative urine culture assessed the maternal bladder microbiota with comparisons made to standard urine culture results. Results Enhanced quantitative urine culture and 16S rRNA gene sequencing detected bacteria in the majority of participants. Lactobacillus and Gardnerella were the most commonly detected microbes. In contrast, standard urine culture had a 100% false-negative rate and failed to detect several known or emerging urinary pathogens. Conclusion There are live bacteria in the bladders of most pregnant women. This challenges the definition of asymptomatic bacteriuria.