BORDETELLA-PERTUSSIS ADENYLATE-CYCLASE - EFFECTS OF AFFINITY-PURIFIED ADENYLATE-CYCLASE ON HUMAN POLYMORPHONUCLEAR LEUKOCYTE FUNCTIONS
BORDETELLA-PERTUSSIS ADENYLATE-CYCLASE - EFFECTS OF AFFINITY-PURIFIED ADENYLATE-CYCLASE ON HUMAN POLYMORPHONUCLEAR LEUKOCYTE FUNCTIONS
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DOI：
10.1128/iai.55.1.135-140.1987
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发表时间：
1987-01-01
影响因子：
3.1
通讯作者：
GALGIANI, JN
中科院分区：
文献类型：
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作者：
FRIEDMAN, RL;FIEDERLEIN, RL;GALGIANI, JN
经亲和纯化的百日咳博德特氏菌腺苷酸环化酶 (AC)，不含污染性百日咳毒素，经证明对人多形核白细胞 (PMN) 具有生物效应。 25和50μg/ml剂量的AC分别使吞噬细胞中的细胞内cAMP水平比基础水平增加7.6至23.5倍。 AC 以剂量依赖性方式抑制 PMN 化学发光、趋化性和超氧化物产生。趋化性和化学发光的50％抑制剂量为36.5μg/ml；对于超氧化物生成，其为71.0μg/ml。尽管这些中性粒细胞代谢功能受损，但没有观察到对吞噬细胞活性的影响。
Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrated to have biological effects on human polymorphonuclear leukocytes (PMN). AC at doses of 25 and 50 .mu.g/ml increased intracellular cAMP levels in the phagocytes 7.6- to 23.5-fold, respectively, above basal levels. AC inhibited PMN chemiluminescence, chemotaxis, and superoxide production in a dose-dependent manner. The 50% inhibitory dose for chemotaxis and chemiluminescence was 36.5 .mu.g/ml; for superoxide generation it was 71.0 .mu.g/ml. Although these PMN metabolic functions were impaired, no effect on phagocytic activity was observed.