Bending rigidities of cell surface molecules P-selectin and PSGL-1
Bending rigidities of cell surface molecules P-selectin and PSGL-1
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DOI：
10.1016/j.jbiomech.2008.11.020
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发表时间：
2009-02-09
影响因子：
2.4
通讯作者：
Zhu, Cheng
中科院分区：
文献类型：
--
作者：
Fang, Ying;Wu, Jianhua;Zhu, Cheng
p -选择素是一种在活化的内皮细胞和血小板上表达的细胞粘附分子。p -选择素糖蛋白配体1 （PSGL-1）是一种在白细胞上表达的粘蛋白。p -选择素和PSGL-1的相互作用介导白细胞在血管表面的聚集和滚动，这是炎症和血栓形成过程中的启动事件。在循环的血流动力学环境中，p -选择素和PSGL-1受到大范围的力的作用，从而导致变形。为了使p -选择素/PSGL-1相互作用在物理上成为可能，这些分子可能需要投射到各自细胞表面的糖萼层之上，这表明它们要么比糖萼的厚度长，要么比糖萼更能支持压缩。因此，这些分子的力学性质及其功能意义值得研究。本文报道了通过分析p -选择素和PSGL-1的热激发曲率波动（其值为100 pN nm数量级）来确定其弯曲刚度的方法(2)。(C) 2008 Elsevier Ltd版权所有。
P-selectin is a cell adhesion molecule expressed on activated endothelial cells and platelets. P-selectin glycoprotein ligand 1 (PSGL-1) is a mucin expressed on leukocytes. The interaction of P-selectin and PSGL-1 mediates leukocyte tethering to and rolling on the vascular surface, which are initiating events in inflammatory and thrombotic processes. In the hemodynamic environment of the circulation, P-selectin and PSGL-1 are Subject to a wide range of forces, which can cause deformation. For P-selectin/PSGL-1 interaction to be physically possible, these molecules may need to project above much of the glycocalyx layers of the respective cell surfaces, suggesting that they are either longer than the thickness of glycocalyx or better able to support compression than the glycocalyx. As such, the mechanical properties of these molecules and their functional implications merit investigation. Here we report determination of the bending rigidities of P-selectin and PSGL-1 by analyzing their thermally excited curvature fluctuations, whose values are of the order of magnitude of 100 pN nm(2). (C) 2008 Elsevier Ltd. All rights reserved.