Effects of hTERT immortalization on osteogenic and adipogenic differentiation of dental pulp stem cells.
Effects of hTERT immortalization on osteogenic and adipogenic differentiation of dental pulp stem cells.
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DOI：
10.1016/j.dib.2016.01.009
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发表时间：
2016-03
期刊：
影响因子：
1.2
通讯作者：
Miranda-Carboni GA
中科院分区：
文献类型：
--
作者：
Ikbale el-A;Goorha S;Reiter LT;Miranda-Carboni GA
这些数据涉及人牙髓干细胞（DPSC）的分化和通过成骨谱系和成脂谱系组成型表达人端粒酶逆转录酶（hTERT）而永生化的DPSC（即，从这些牙髓干细胞制备骨产生细胞和脂肪产生细胞）。这些数据补充了另一项研究，以表征永生化DPSC用于神经遗传性疾病的研究“表征来自永生化牙髓干细胞的神经元用于神经遗传性疾病的研究”。本研究中使用了一个典型对照细胞系的两个拷贝（技术重复）。数据表示原代DPSC向成骨细胞的分化比hTERT永生化DPSC有效约60%。相反，原代和永生化DPSC都很难分化成脂肪细胞。显示了早期和晚期脂肪形成和成骨基因标志物的mRNA表达水平。
These data relate to the differentiation of human dental pulp stem cells (DPSC) and DPSC immortalized by constitutively expressing human telomerase reverse transcriptase (hTERT) through both osteogenic and adipogenic lineages (i.e. to make bone producing and fat producing cells from these dental pulp stem cells). The data augment another study to characterize immortalized DPSC for the study of neurogenetic “Characterization of neurons from immortalized dental pulp stem cells for the study of neurogenetic disorders”. Two copies of one typical control cell line (technical replicates) were used in this study. The data represent the differentiation of primary DPSC into osteoblast cells approximately 60% more effectively than hTERT immortalized DPSC. Conversely, both primary and immortalized DPSC are poorly differentiated into adipocytes. The mRNA expression levels for both early and late adipogenic and osteogenic gene markers are shown.