Electron microscopical studies on axonal degeneration in the rat iris following ganglionectomy.
Electron microscopical studies on axonal degeneration in the rat iris following ganglionectomy.
复制标题
神经节切除术后大鼠虹膜轴突变性的电子显微镜研究。
DOI：
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发表时间：
1969
影响因子：
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通讯作者：
K. C. Richardson
中科院分区：
文献类型：
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作者：
C. D. Roth;K. C. Richardson
颈上神经节切除可导致大鼠虹膜内肾上腺素能轴突在术后24小时内迅速而完全地消失。术后48小时，经四氧化三铯固定后，虹膜的电子显微镜下形态正常，因为碎片已经消失，自主神经丛中大量存活的胆碱能轴突给人的印象是总的神经支配仍然完好无损。然而，高锰酸盐固定术后48小时至一年的失神经虹膜轴突中缺乏颗粒状小泡，在这一方面与正常不同。睫状神经节切除后，同一类型的单个轴突变性在24小时内开始，但持续数天，涉及的轴突数量比上次手术更多。切除这两组神经节会加剧轴突的丧失，并导致虹膜组织的一些萎缩。然而，表面上完好的轴突残留物会持续长达10个月之久。以往在光镜下神经节切除对虹膜神经支配的结果是不一致的，这是由于技术上的不足，加上在早期的文献中，并不知道在虹膜神经丛的所有部分都有肾上腺素能和胆碱能轴突的紧密混合。
Superior cervical ganglionectomy causes a very rapid and complete distintegration of adrenergic axons in the rat iris commencing less than 24 hours after operation. At 48 hours post-operatively the electronmicroscopical appearance of the iris is normal with osmium tetroxide fixation because the debris has vanished and the abundant surviving cholinergic axons in the autonomic ground plexus give the impression that the total innervation is still intact. However, with permanganate fixation the denervated iris examined 48 hours to one year after operation lacks granular vesicles in its axons and appears in this single respect different from normal. After ciliary ganglionectomy the same kind of degeneration of individual axons begins within 24 hours, but continues for several days, involving a greater number of axons than the previous operation. Removal of both sets of ganglia intensifies the loss of axons and causes some atrophy of the iris tissues. A residue of apparently intact axons persists, however, for as long as ten months. The previously discordant results of ganglionectomy on iris innervation obtained by light microscopy are shown to be dependent on technical inadequacies, combined with the fact that in the earlier literature it was not known that there is a close intermingling of adrenergic and cholinergic axons in all parts of the iris plexuses.