Chlamydomonas reinhardtii: the model of choice to study mitochondria from unicellular photosynthetic organisms.
Chlamydomonas reinhardtii: the model of choice to study mitochondria from unicellular photosynthetic organisms.
复制标题
DOI：
10.1007/978-1-59745-365-3_10
复制
发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Gonzalez-Halphen, Diego
中科院分区：
文献类型：
--
作者：
Funes, Soledad;Franzen, Lars-Gunnar;Gonzalez-Halphen, Diego
莱茵衣藻是研究光合作用、细胞分裂、鞭毛生物发生以及最近的线粒体功能的模式生物。与高等植物相比，它具有明显的优势，因为它是单细胞、单倍体，易于四分体分析，并且它的三个基因组经过特定的转化。它还可以在醋酸盐上进行光自养或异养生长，使得光合机器的组装对于细胞活力来说不是必需的。开发的方法允许分离不含类囊体污染物的莱茵衣藻线粒体。我们回顾了用于绿藻线粒体生化表征的一般程序。
Chlamydomonas reinhardtii is a model organism to study photosynthesis, cellular division, flagellar biogenesis, and, more recently, mitochondrial function. It has distinct advantages in comparison to higher plants because it is unicellular, haploid, and amenable to tetrad analysis, and its three genomes are subject to specific transformation. It also has the possibility to grow either photoautotrophically or heterotrophically on acetate, making the assembly of the photosynthetic machinery not essential for cell viability. Methods developed allow the isolation of C. reinhardtii mitochondria free of thylakoid contaminants. We review the general procedures used for the biochemical characterization of mitochondria from this green alga.