Dragon's Blood Inhibits Chronic Inflammatory and Neuropathic Pain Responses by Blocking the Synthesis and Release of Substance P in Rats
Dragon's Blood Inhibits Chronic Inflammatory and Neuropathic Pain Responses by Blocking the Synthesis and Release of Substance P in Rats
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DOI：
10.1254/jphs.11160fp
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发表时间：
2012-01-01
影响因子：
3.5
通讯作者：
Tang, He-Bin
中科院分区：
文献类型：
--
作者：
Li, Yu-Sang;Wang, Jun-Xian;Tang, He-Bin
作为一种传统中药，龙血（DB）因其强大的抗炎和镇痛作用而被广泛用于治疗各种疼痛，已有数千年的历史。在本研究中，我们观察到，灌胃剂量为 0.14、0.56 和 1.12 g/kg 的 DB 分别有效抑制角叉菜胶内伤或坐骨神经损伤（慢性缩窄性损伤）大鼠的足爪水肿、痛觉过敏、环氧合酶-2 (COX-2) 蛋白表达或前速激肽-A mRNA 表达。将培养的大鼠背根神经节 (DRG) 神经元短期（15 秒或 10 分钟）预暴露于 DB（0.3、3 和 30 μg/ml）或其成分交趾黄素 B（CB；0.1、1 和 10 μM）可阻断辣椒素引起的细胞内钙离子浓度和 P 物质释放的增加。此外，培养的大鼠 DRG 神经元长期（180 分钟）暴露于 DB 或 CB 显着减弱了缓激肽诱导的 P 物质释放。这些发现表明，DB通过抑制COX-2蛋白诱导和细胞内钙离子浓度来阻断P物质的合成和释放，从而发挥抗炎和镇痛作用。因此，DB可能成为治疗慢性疼痛的一种有前途的有效治疗剂，其有效成分CB可能部分有助于抗炎和镇痛作用。
As a traditional Chinese medicine, dragon's blood (DB) is widely used in treating various pains for thousands of years due to its potent anti-inflammatory and analgesic effects. In the present study, we observed that intragastric administration of DB at dosages of 0.14, 0.56, and 1.12 g/kg potently inhibited paw edema, hyperalgesia, cyclooxygenase-2 (COX-2) protein expression, or preprotachykinin-A mRNA expression in carrageenan-intlamed or sciatic nerve injured (chronic constriction injury) rats, respectively. A short-term (15 s or 10 min) pre-exposure of cultured rat dorsal root ganglion (DRG) neurons to DB (0.3, 3, and 30 mu g/ml) or its component cochinchinenin B (CB; 0.1, 1, and 10 mu M) blocked capsaicin-evoked increases in both the intracellular calcium ion concentration and the substance P release. Moreover, a long-term (180 min) exposure of cultured rat DRG neurons to DB or CB significantly attenuated bradykinin-induced substance P release. These findings indicate that DB exerts anti-inflammatory and analgesic effects by blocking the synthesis and release of substance P through inhibition of COX-2 protein induction and intracellular calcium ion concentration. Therefore, DB may serve as a promising potent therapeutic agent for treatment of chronic pain, and its effective component CB might partly contribute to anti-inflammatory and analgesic effects.