ESTROGENIC CONTROL OF MONOAMINE-OXIDASE-A ACTIVITY IN HUMAN NEUROBLASTOMA-CELLS EXPRESSING PHYSIOLOGICAL CONCENTRATIONS OF ESTROGEN-RECEPTOR
ESTROGENIC CONTROL OF MONOAMINE-OXIDASE-A ACTIVITY IN HUMAN NEUROBLASTOMA-CELLS EXPRESSING PHYSIOLOGICAL CONCENTRATIONS OF ESTROGEN-RECEPTOR
复制标题
DOI：
10.1016/0014-2999(95)00387-z
复制
发表时间：
1995-09-15
影响因子：
5
通讯作者：
MAGGI, A
中科院分区：
文献类型：
--
作者：
MA, ZQ;VIOLANI, E;MAGGI, A
有几条证据支持雌激素在女性情感障碍表现中所起作用的假设。对一些抗抑郁药物的作用机制的分析清楚地表明，这些复杂的行为病理的病因学中的儿茶酚胺能系统的参与。因此，目前在体外研究进行了调查雌激素和儿茶酚胺代谢的神经源性细胞之间的功能联系的存在。使用的模型系统是通过稳定转染雌激素受体cDNA（SK-ER 3）获得的人神经母细胞瘤细胞系。本研究表明，在SK-ER 3雌激素受体的激活与单胺氧化酶A活性的显着下降。在用生理浓度的17 β-雌二醇处理后观察到这种作用，并且可以被类固醇受体的特异性拮抗剂ICI 182，780阻断。双丁酰环腺苷酸作用，像雌激素一样，对SK-ER 3细胞的分化状态不影响单胺氧化酶A的活性。本研究提供了强有力的证据，雌激素受体和单胺氧化酶A活性在人类神经源性细胞之间的严格关系，从而有利于通过抑制单胺氧化途径发挥雌激素的抗抑郁作用的假设。
Several lines of evidence support the hypothesis of a role played by estrogens in the manifestation of affective disorders in women. The analysis of the mechanism of action of a number of antidepressant drugs clearly demonstrated the involvement of the catecholaminergic system in the etiology of these complex behavioral pathologies. The present in vitro study was therefore undertaken to investigate the presence of a functional link between estrogen and catecholamine metabolism in cells of neural origin. The model system utilized was a human neuroblastoma cell line which was obtained by stable transfection of the estrogen receptor cDNA (SK-ER3). The present study shows that in SK-ER3 activation of the estrogen receptor correlates with a marked decrease in monoamine oxidase A activity. This effect is observed following treatment with a physiological concentration of 17 beta-estradiol and can be blocked by the specific antagonist of the steroid receptor, ICI 182,780. Dibutyryl cyclic AMP acting, like estrogens, on the state of differentiation of SK-ER3 cells did not affect monoamine oxidase A activity. The present study provides strong evidence of a strict relationship between estrogen receptor and monoamine oxidase A activity in human cells of neural origin, thus favoring the hypothesis of an antidepressive effect of estrogens exerted via inhibition of the monoamine oxidative pathway.