Population structure and reproductive mode of Dothistroma septosporum in the Brittany peninsula of France
Population structure and reproductive mode of Dothistroma septosporum in the Brittany peninsula of France
复制标题
法国布列塔尼半岛 Dothistroma septosporum 种群结构及繁殖方式
DOI：
10.1007/s10658-015-0678-8
复制
发表时间：
2015
影响因子：
1.8
通讯作者：
I. Barnes
中科院分区：
文献类型：
--
作者：
M. Mullett;A. Brown;I. Barnes
针叶枯病 (DNB) 被认为是松树最严重的叶部病害之一，自 20 世纪 90 年代末以来对法国的松树种植园造成了严重损害。使用物种特异性交配型引物和微卫星标记，在法国布列塔尼的六个地点对 DNB 的病原体之一 D. septosporum 的遗传多样性、繁殖策略和种群结构进行了研究。开发了多重 PCR 测定法，以有效且高效地筛选获得的分离株上的 11 种可用微卫星标记。从分析的 282 个分离株中，鉴定出 81 个独特的多位点单倍型，表明这些位点的病原体具有多样性（单倍型多样性范围为 0.403 至 0.962）但高度克隆的结构。尽管所有位点都包含两种交配类型，但随机交配被克隆校正和非克隆校正数据集上的关联索引和简约树长度排列测试拒绝，但受到克隆校正数据集中的交配类型比率支持。使用 STRUCTURE 确定了四个人口集群。地点和寄主物种在布列塔尼的种群结构中都没有发挥重要作用。这些发现，以及在不同地点和不同宿主上观察到的相同单倍型，表明病原体在半岛内移动。总体而言，发现病原体的种群结构受到有性重组的影响，但在位点内克隆繁殖占主导地位。
Dothistroma needle blight (DNB) is regarded as one of the most significant foliar diseases of pine and has caused significant damage to pine plantations in France since the late 1990s. The genetic diversity, reproductive strategy and population structure of D. septosporum, one of the causal agents of DNB, was investigated at six sites across Brittany, France, using species-specific mating type primers and microsatellite markers. Multiplex PCR assays were developed to effectively and efficiently screen 11 of the available microsatellite markers on the isolates obtained. From 282 isolates analysed, 81 unique multilocus haplotypes were identified, indicating a diverse (haplotypic diversity ranged from 0.403 to 0.962) yet highly clonal structure of the pathogen at these sites. Although all sites contained both mating types, random mating was rejected by the index of association and parsimony tree length permutation tests on both clone-corrected and non-clone-corrected datasets but supported by mating type ratios in the clone-corrected datasets. Four population clusters were identified using STRUCTURE. Neither locality nor host species played a significant role in the population structure in Brittany. These findings, along with the observation of identical haplotypes at different sites and on different hosts, suggest movement of the pathogen within the peninsula. Overall, the pathogen’s population structure was found to be influenced by sexual recombination yet within sites clonal reproduction predominated.
使用多位点基因型数据推断群体结构：连锁位点和相关等位基因频率。
影响因子：
3.3
作者：
Falush,Daniel;Stephens,Matthew;Pritchard,JonathanK
通讯作者：
Pritchard,JonathanK