Quantitative analysis of urea in serum by synchronous modulation and demodulation fluorescence spectroscopy
Quantitative analysis of urea in serum by synchronous modulation and demodulation fluorescence spectroscopy
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同步调制解调荧光光谱法定量分析血清中的尿素
DOI：
10.1016/j.saa.2021.120645
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Ling Lin
中科院分区：
文献类型：
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作者：
Kang Wang;Shaohua Wu;Jing Zhao;Mei Zhou;Gang Li;Dan Wang;Ling Lin
高精度光谱数据是复杂溶液组分定量分析的必要前提。为了提高频谱数据的精度，本文提出了一种同步调制解调的方法。本文还结合“M+ N”理论，巧妙地利用血清中各组分的激发荧光及其自吸收现象，采集血清样品的荧光光谱，然后采用偏最小二乘法（PLS）和三次优化模型法建立模型，对血清中尿素浓度进行分析。同时，为了验证同步调制解调方法的有效性，利用未调制的荧光光谱建立了尿素浓度的回归模型。与未调制的荧光光谱建模结果相比，调制解调后的荧光光谱建模结果有了明显的改善。同步调制解调后荧光光谱的建模结果中，Rc = 0.916753，RMSEC = 2.05848 mmol/L，Rp = 0.79663，RMSEP = 3.16812 mmol/L，Rp-al 1 = 0.88879，RMSEP-al 1 = 2.32114 mmol/L。结果表明，本文提出的同步调制解调方法不仅降低了暗电流、环境光和背景噪声对光谱数据信噪比的影响，而且有效避免了斩波器和光谱仪不同步带来的误差。因此，本文所采用的方法不仅提高了光谱数据的信噪比和准确性，而且提高了复杂溶液光谱定量分析的准确性。
High-precision spectral data is a necessary prerequisite for quantitative analysis of complex solution components. In order to improve the accuracy of spectral data, this paper proposes a method of synchronous modulation and demodulation. This article also combines the “M+ N” theory, cleverly uses the excitation fluorescence of the components in the serum and its self-absorption phenomenon, collects the fluorescence spectrum of the serum sample, and then uses the partial least squares (PLS) method and the cubic optimization model method to establish a model to analyze the urea concentration of serum. At the same time, in order to verify the effectiveness of synchronous modulation and demodulation method, the unmodulated fluorescence spectrum is used to establish the regression model of urea concentration. Compared with the unmodulated fluorescence spectrum modeling results, the fluorescence spectrum modeling results after modulation and demodulation have been significantly improved. In the modeling results of fluorescence spectrum after synchronous modulation and demodulation, the R c is 0.916753, the RMSEC is 2.05848 mmol/L, the R p is 0.79663, and the RMSEP is 3.16812 mmol/L, the R p-a l l is 0.88879, and the RMSEP-all is 2.32114 mmol/L. The results show that the method of synchronous modulation and demodulation proposed in this paper not only reduces the influence of dark current, ambient light and background noise on the signal-to-noise ratio of the spectral data, but also effectively avoids the error caused by the non-synchronization of the chopper and the spectrometer. Therefore, the method used in this paper not only improves the signal-to-noise ratio and accuracy of spectral data, but also improves the accuracy of spectral quantitative analysis of complex solutions.