Exploiting salivary miR-31 as a clinical biomarker of oral squamous cell carcinoma
Exploiting salivary miR-31 as a clinical biomarker of oral squamous cell carcinoma
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DOI：
10.1002/hed.21713
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发表时间：
2012-02-01
影响因子：
2.9
通讯作者：
Chang, Kuo-Wei
中科院分区：
文献类型：
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作者：
Liu, Chung-Ji;Lin, Shu-Chun;Chang, Kuo-Wei
口腔癌是世界范围内一种重要的恶性肿瘤。MicroRNAs （miRNAs）是内源性表达的非编码rna，可调节靶向mrna的转录后水平。MiRNA-31（miR-31）在口腔癌组织中显著上调，在口腔癌发生中起致癌作用。我们通过定量逆转录聚合酶链反应（RTPCR）分析了口腔癌（n = 45）、口腔疣状白斑（n = 10）和对照健康个体（n = 24）唾液中miR-31的水平。在口腔癌患者的所有临床阶段，包括非常小的肿瘤，唾液miR-31均显著升高。然而，我们的初步分析显示，与对照组相比，口腔疣状白斑患者的唾液miR-31没有增加。miR-31在唾液中的含量高于血浆，表明唾液中的miR-31是口腔恶性肿瘤更敏感的标志物。口腔癌切除后，唾液miR-31明显降低，说明上调的唾液miR-31大部分来自肿瘤组织。我们的研究结果指出了唾液miR-31作为口腔癌早期检测和术后随访的生物标志物的潜在应用。(C) 2011 Wiley期刊公司中国生物医学工程学报（英文版），2012
Background Oral carcinoma is an important malignancy throughout the world. MicroRNAs (miRNAs) are endogenously expressed, non-coding RNAs that regulate post-transcriptional levels of targeted mRNAs. MiRNA-31(miR-31) is significantly upregulated in oral carcinoma tissues and plays oncogenic roles in oral carcinogenesis.Methods. We analyzed the levels of miR-31 in saliva of patients with oral carcinoma (n = 45), oral verrucous leukoplakia (n = 10), and control healthy individuals (n = 24) by quantitative reverse transcriptase-polymerase chain reaction (RTPCR).Results. Salivary miR-31 was significantly increased in patients with oral carcinoma at all clinical stages, including very small tumors. However, our preliminary analysis showed no increase of salivary miR-31 in patients with oral verrucous leukoplakia relative to controls. The miR-31 was more abundant in saliva than in plasma, suggesting salivary miR-31 was a more sensitive marker for oral malignancy. After excision of oral carcinoma, salivary miR-31 was remarkably reduced, indicating that most of the upregulated salivary miR-31 came from tumor tissues.Conclusion. Our results point to a potential application of salivary miR-31 as a biomarker for early detection and postoperative follow-up of oral carcinoma. (C) 2011 Wiley Periodicals, Inc. Head Neck 34: 219-224, 2012