Development and evaluation of biocompatible films of polytetrafluoroethylene polymers holding lithium phthalocyanine crystals for their use in EPR oximetry
Development and evaluation of biocompatible films of polytetrafluoroethylene polymers holding lithium phthalocyanine crystals for their use in EPR oximetry
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DOI：
10.1016/j.bios.2005.03.009
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发表时间：
2006-01-15
影响因子：
12.6
通讯作者：
Gallez, B
中科院分区：
文献类型：
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作者：
Dinguizli, A;Jeumont, S;Gallez, B
电子顺磁共振（EPR）血氧仪是一项功能强大的技术，可以监测组织中的氧合。组织氧合的测量可以实现使用酞菁锂（LiPc）晶体作为氧报告。为了使传感系统具有生物相容性并确保系统响应性的长期稳定性，我们开发了嵌入LiPc晶体的Teflon AF 2400（(R)）薄膜。这些系统可以用作可回收的插入物或植入式谐振器或导管的一部分。原子力显微镜研究表明，膜的表面是规则的、平面的。植入小鼠后，传感器对氧的响应（EPR线宽与pO(2)的关系）保持不变，不受灭菌或辐照的影响。使用谐振器，将LiPc嵌入Teflon AF 2400中，植入家兔的腓肠肌，可以在几周内监测氧气。几项试验也证明了该系统的生物相容性：(1)没有发现溶血作用；(2)对提取物进行全身注射试验，未发现毒性；(3)植入1周或3个月后兔肌肉组织学分析与标准聚乙烯生物相容性装置相似。这些先进的氧传感器是未来EPR血氧仪临床前和临床开发的有前途的工具。这些进展可以应用于需要透氧膜的生物传感器的其他应用。(c) 2005 Elsevier B.V.版权所有
Electron paramagnetic resonance (EPR) oximetry is a powerful technology that allows the monitoring of oxygenation in tissues. The measurement of tissue oxygenation can be achieved using lithium phthalocyanine (LiPc) crystals as oxygen reporters. In order to have biocompatibility for the sensing system and to assure long-term stability in the responsiveness of the system, we developed films of Teflon AF 2400((R)) with embedded LiPc crystals. These systems can be used as retrievable inserts or parts of an implantable resonator or catheter. Atomic force microscopy studies revealed that the surface of the films was regular and planar. The response to oxygen of the sensor (EPR linewidth as a function Of pO(2)) remained unchanged after implantation in mice, and was not affected by sterilization or irradiation. The use of resonators, holding LiPc embedded in Teflon AF 2400, implanted in the gastrocnernius muscle of rabbits allowed the monitoring of oxygen during several weeks. Several assays also demonstrated the biocompatibility of the system: (1) no hemolytic effect was noted; (2) no toxicity was found using the systemic injection test of extracts; (3) histological analysis in rabbit muscle in which the films were implanted for I week or 3 months was similar to standard polyethylene biocompatible devices. These advanced oxygen sensors are promising tools for future pre-clinical and clinical developments of EPR oximetry. These developments can be applied for other applications of biosensors where there is a need for oxygen permeable membranes. (c) 2005 Elsevier B.V. All rights reserved.