Impact of DAG stimulation on mineral synthesis, mineral structure and osteogenic differentiation of human cord blood stem cells
Impact of DAG stimulation on mineral synthesis, mineral structure and osteogenic differentiation of human cord blood stem cells
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DOI：
10.1016/j.scr.2011.09.004
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发表时间：
2012-03-01
影响因子：
1.2
通讯作者：
Handschel, Joerg
中科院分区：
文献类型：
--
作者：
Lammers, Lydia;Naujoks, Christian;Handschel, Joerg
地塞米松、抗坏血酸和β-甘油磷酸盐（DAG）的成骨刺激以何种方式影响矿化过程、组装矿物质的组成和结构尚未探索，因此，我们分析和表征了DAG刺激和未刺激的3D人类非限制性体干细胞（USSC）培养物中的生物矿化。采用组织学染色、扫描电镜（SEM）、半定量能量色散X射线能谱（EDX）、定量波长色散X射线能谱（WDX）、透射电镜（TEM）、选区电子衍射（SAED）和拉曼光谱等方法对微球进行分析，并通过扫描电镜和组织学染色检测两组微球中的矿物质成分，发现两组微球结构存在差异。DAG影响了USCs的分化及矿化物的形成、结构和组成，扫描电镜显示+DAG球内的细胞具有成骨样细胞的形态特征。EDX和WDX证实了两组中的Ca-P矿物质。总体而言，发现的矿物质显示出与骨材料矿物质的结构相似性。(C)2011 Elsevier B. V.保留所有权利。
It remains unexplored in what way osteogenic stimulation with dexamethasone, ascorbic acid and beta-glycerol phosphate (DAG) influences the process of mineralization, the composition and structure of the assembled mineral. Therefore, we analyzed and characterized biomineralization in DAG-stimulated and unstimulated 3D human unrestricted somatic stem cell (USSC) cultures. Microspheres were analyzed by histological staining, scanning electron microscopy (SEM), semi-quantitative energy-dispersive X-ray spectroscopy (EDX), quantitative wavelength-dispersive X-ray spectroscopy (WDX), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and Raman spectroscopy.Mineral material was detected by SEM and histological staining in both groups, and showed structural differences. DAG influenced the differentiation of USSCs and the formation, structure and composition of the assembled mineral. SEM showed that cells of the +DAG spheres exhibited morphological signs of osteoblast-like cells. EDX and WDX confirmed a Ca-P mineral in both groups. Overall, the mineral material found showed structural similarities to the mineral substance of bony material. (C) 2011 Elsevier B.V. All rights reserved.