Expression of neurotrophin and its tropomyosin-related kinase receptors (Trks) during axonal regeneration following spinal cord injury in larval lamprey.
Expression of neurotrophin and its tropomyosin-related kinase receptors (Trks) during axonal regeneration following spinal cord injury in larval lamprey.
复制标题
七鳃鳗幼虫脊髓损伤后轴突再生过程中神经营养素及其原肌球蛋白相关激酶受体 (Trks) 的表达。
DOI：
10.1016/j.neuroscience.2011.03.024
复制
发表时间：
2011
期刊：
影响因子：
3.3
通讯作者：
Shifman,MI
中科院分区：
文献类型：
--
作者：
Laramore,C;Maymind,E;Shifman,MI
Exogenous neurotrophins reduce neuronal atrophy and promote regeneration following spinal cord injury but little is known about the endogenous expression of neurotrophins and their tropomyosin-related kinase (Trk) receptors in the injured spinal cord. For this purpose, we used the larval lamprey because it recovers from complete spinal transection and axons regenerate selectively in their correct paths. We cloned lamprey neurotrophin (NT) and its two Trk receptors and assessed their mRNA expression byin situhybridization and QRT-PCR in control animals and after spinal cord transection. Control lampreys showed a longitudinal array of NT-expressing neurons along length of the spinal cord. At 2 weeks post-transection, NT expression was downregulated in neurons close to the transection, but was little affected remote from the lesion. By 4 weeks, NT expression returned to control levels in spinal cord neurons rostral and caudal to the lesion, although it was upregulated in reactive microglia at 14 and 30 days post-transection. Double-labelin situhybridization for Trk1 and Trk2 showed that Trk transcripts were expressed in several giant reticulospinal neurons, including the Mauthner neurons. After spinal cord transection, Trk1 mRNA expression was downregulated, but Trk2 mRNA expression was not changed or was increased. Thus, our data suggest that spinal cord injury in larval lampreys modulate expression of endogenous neurotrophin and induces proliferation of macrophage/microglial cells that express neurotrophin.
