An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice.
An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice.
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DOI：
10.1038/s41589-020-00660-y
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发表时间：
2021-01
影响因子：
14.8
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zhang JF;Liu B;Hong I;Mo A;Roth RH;Tenner B;Lin W;Zhang JZ;Molina RS;Drobizhev M;Hughes TE;Tian L;Huganir RL;Mehta S;Zhang J
Protein kinases control nearly every facet of cellular function. These key signaling nodes integrate diverse pathway inputs to regulate complex physiological processes, and aberrant kinase signaling is linked to numerous pathologies. While fluorescent protein-based biosensors have revolutionized the study of kinase signaling by allowing direct, spatiotemporally precise kinase activity measurements in living cells, powerful new molecular tools capable of robustly tracking kinase activity dynamics across diverse experimental contexts are needed to fully dissect the role of kinase signaling in physiology and disease. Here, we report the development of an ultrasensitive, second-generation excitation-ratiometric protein kinase A (PKA) activity reporter (ExRai-AKAR2), obtained via high-throughput linker library screening, that enables sensitive and rapid monitoring of live-cell PKA activity across multiple fluorescence detection modalities, including plate reading, cell sorting and one- or two-photon imaging. Notably, in vivo visual cortex imaging in awake mice reveals highly dynamic neuronal PKA activity rapidly recruited by forced locomotion. Further information on research design is available in the Nature Research Reporting Summary linked to this article.
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影响因子：
16.2
作者：
Chen Y;Granger AJ;Tran T;Saulnier JL;Kirkwood A;Sabatini BL
通讯作者：
Sabatini BL
影响因子：
4.6
作者：
Carnegie, Graeme K.;Means, Christopher K.;Scott, John D.
通讯作者：
Scott, John D.
DOI：
10.1073/pnas.0712008105
发表时间：
2008-03-18
影响因子：
11.1
作者：
Hires, Samuel Andrew;Zhu, Yongling;Tsien, Roger Y.
通讯作者：
Tsien, Roger Y.
影响因子：
--
作者：
Depry, Charlene;Allen, Michael D.;Zhang, Jin
通讯作者：
Zhang, Jin
影响因子：
0.8
作者：
Drobizhev, Mikhail;Molina, Rosana S.;Hughes, Thomas E.
通讯作者：
Hughes, Thomas E.