In vivo bioimaging using photogenic rats:: Fate of injected bone marrow-derived mesenchymal stromal cells
In vivo bioimaging using photogenic rats:: Fate of injected bone marrow-derived mesenchymal stromal cells
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DOI：
10.1016/j.jaut.2007.12.007
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发表时间：
2008-05-01
影响因子：
12.8
通讯作者：
Kobayashi, Eiji
中科院分区：
文献类型：
--
作者：
Hara, Mayumi;Murakami, Takashi;Kobayashi, Eiji
骨髓来源的间充质基质细胞(MSCs)具有自我更新和分化的能力，可以分化为肌肉、骨、脂肪或软骨细胞。基于这一潜力和可行性，骨髓间充质干细胞有望用于人类疾病的细胞治疗。有趣的是，MSCs在体内迁移到不同的位置，包括损伤和肿瘤部位。然而，它们的细胞命运和分布仍不清楚。在这篇综述中，我们首先描述了表达荧光和/或发光蛋白(如绿色荧光蛋白和荧光素酶)的上光转基因大鼠的潜力，然后重点介绍了MSCs向损伤和肿瘤部位的特征迁移。此外，我们还将讨论针对受伤部位的有效投放方法。与光学成像的现代进步相结合，上光大鼠系统提供了创新的临床前工具和一个新的平台，在此基础上进一步了解与干细胞生物学有关的问题。(C)2007爱思唯尔有限公司。保留所有权利。
Mesenchymal stromal cells (MSCs) derived from bone marrow have the capacity for self-renewal and differentiation, and can give rise to cells of a muscle, bone, fat or cartilage lineage. Based on this potential and feasibility, MSCs are expected to be used in cell therapy for human diseases. Intriguingly, MSCs migrate to various in vivo locations, including injury and tumor sites. However, their cellular fate and distribution remain unclear. In this review, we first describe the potential of a photogenic transgenic rat that expresses fluorescent and/or luminescent proteins (e.g., green fluorescent protein and luciferase), and then focus on the characteristic migration of MSCs to injury and tumor sites. In addition, we will discuss an efficient delivery method for targeting the injured site. Synergized with modem advances in optical imaging, the photogenic rat system provides innovative preclinical tools and a new platform on which to further our understanding of matters concerning stem cell biology. (C) 2007 Elsevier Ltd. All rights reserved.