Suppression of MyoD induces spontaneous adipogenesis in skeletal muscle progenitor cell culture.
Suppression of MyoD induces spontaneous adipogenesis in skeletal muscle progenitor cell culture.
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DOI：
10.1111/asj.13573
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
K. Yamanouchi;Katsuyuki Nakamura;S. Takeuchi;Tohru Hosoyama;T. Matsuwaki;M. Nishihara
中科院分区：
文献类型：
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作者：
K. Yamanouchi;Katsuyuki Nakamura;S. Takeuchi;Tohru Hosoyama;T. Matsuwaki;M. Nishihara
肌内脂肪组织堆积程度是影响肉品质的因素之一。在肌肉萎缩症和肌肉减少症等骨骼肌病理状态下也观察到脂肪细胞的积累。骨骼肌中脂肪细胞的起源是间充质祖细胞，它可以产生脂肪细胞和成纤维细胞。在本研究中，我们证明，在大鼠骨骼肌祖细胞培养物（包括肌源性卫星细胞和间充质祖细胞）中，siRNA介导的MyoD表达抑制导致肌管形成减少和白色脂肪细胞意外自发出现。抑制 myomaker 表达也会导致肌管形成完全消失，而不减少 MyoD 表达，但在这种情况下没有观察到脂肪形成，这表明 MyoD 表达的下降而不是肌管形成的减少是诱导脂肪形成所必需的。此外，通过抑制培养物中MyoD表达诱导的自发脂肪生成被对照培养物的条件培养基抑制，表明MyoD阳性肌原细胞分泌抗脂肪形成因子。这些结果表明生肌细胞对脂肪生成的调节机制的存在。
The degree of intramuscular adipose tissue accumulation is one of the factors affecting meat quality. Accumulation of adipocytes is also observed under the pathological condition of skeletal muscle such as muscular dystrophy and sarcopenia. The origin of adipocytes seen in skeletal muscle is mesenchymal progenitor cells that can give rise to both adipocytes and fibroblasts. In the present study, we demonstrated that siRNA-mediated suppression of MyoD expression in rat skeletal muscle progenitor cell culture, which comprises both myogenic satellite cells and mesenchymal progenitor cells, resulted in diminished myotube formation and an unexpected spontaneous appearance of white adipocytes. Suppressing myomaker expression also resulted in complete absence of myotube formation without reducing MyoD expression, but no adipogenesis was seen in this scenario, indicating that decline in MyoD expression rather than decreased myotube formation is necessary to induce adipogenesis. In addition, spontaneous adipogenesis induced by suppressing MyoD expression in culture was inhibited by the conditioned medium from control culture, indicating that anti-adipogenic factor(s) are secreted from MyoD-positive myogenic cells. These results indicate the presence of regulatory mechanism on adipogenesis by myogenic cells.