Proteomic biosignatures for monocyte-macrophage differentiation
Proteomic biosignatures for monocyte-macrophage differentiation
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DOI：
10.1016/j.cellimm.2011.07.002
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发表时间：
2011-01-01
影响因子：
4.3
通讯作者：
Gendelman, Howard E.
中科院分区：
文献类型：
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作者：
Kraft-Terry, Stephanie D.;Gendelman, Howard E.
我们使用脉冲稳定同位素标记的氨基酸在细胞培养（pSILAC），以评估蛋白质动力学单核细胞-巨噬细胞分化。pSILAC允许新合成的蛋白质的代谢标记。在培养物中从3天至7天评价这种从头蛋白质产生。通过液相色谱-串联质谱法鉴定蛋白质，然后通过MaxQuant定量。蛋白质-蛋白质连接，然后通过免疫途径分析进行评估。所鉴定的蛋白质与细胞内稳态、自由基清除、分子蛋白质转运、碳水化合物代谢、小分子化学和细胞形态有关。这些数据证明了特定的生物学事件与特定生物系统中的单核细胞转化有关。(C)2011 Elsevier Inc. All rights reserved.
We used pulsed stable isotope labeling of amino acids in cell culture (pSILAC) to assess protein dynamics during monocyte-macrophage differentiation. pSILAC allows metabolic labeling of newly synthesized proteins. Such de novo protein production was evaluated from 3 to 7 days in culture. Proteins were identified by liquid chromatography-tandem mass spectrometry then quantified by MaxQuant. Protein-protein linkages were then assessed by Ingenuity Pathway Analysis. Proteins identified were linked to cell homeostasis, free radical scavenging, molecular protein transport, carbohydrate metabolism, small molecule chemistry, and cell morphology. The data demonstrates specific biologic events that are linked to monocyte transformation in a defined biologic system. (C) 2011 Elsevier Inc. All rights reserved.