Fish oil supplementation of lactating mothers affects cytokine production in 21/2-year-old children
Fish oil supplementation of lactating mothers affects cytokine production in 21/2-year-old children
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DOI：
10.1007/s11745-005-1429-6
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发表时间：
2005-07-01
期刊：
影响因子：
1.9
通讯作者：
Frokiær, H
中科院分区：
文献类型：
--
作者：
Lauritzen, L;Kjær, TMR;Frokiær, H
N-3多不饱和脂肪酸影响免疫功能，并可能影响发育过程中的细胞因子表型。为了研究哺乳期间补充母亲鱼油是否可以改变儿童后来的免疫反应，122名鱼摄入量低于人口中位数的哺乳期丹麦母亲被随机分为两组，在哺乳的前4个月添加4.5g/d鱼油(相当于1.5g/d的n-3长链多不饱和脂肪酸)或橄榄油。53名母亲的鱼摄入量在人口中最高的四分之一也包括在内。分别于术后4个月和21年半测定红细胞膜的脂肪酸组成。在21/2岁时检测血浆免疫球蛋白E(IgE)水平和内毒素刺激的全血培养中细胞因子的产生。鱼油组婴儿红细胞n-3多不饱和脂肪酸在4个月时高于橄榄油组(P<；0.001)，但在21岁半时不再有差异。鱼油组产生的内毒素诱导的干扰素-γ中位数是橄榄油组的4倍(P=0.034)，而白介素10的产生与橄榄油组相似。4个月时，鱼油组干扰素-γ/IL-10比值为对照组的2倍(P=0.019)，且与红细胞20：5n-3/20：4n-6呈正相关(P=0.050)。两组特应性儿童的百分比和血浆IgE没有差异，但这项研究并不是为了观察特应性。与随机组相比，鱼摄入量高的母亲的孩子的细胞因子反应和红细胞FA组成处于中等水平。在哺乳期补充鱼油后2年，儿童的体外干扰素-γ产生增加，这可能反映了免疫系统更快的成熟。
n-3 PUFA influence immune functioning and may affect the cytokine phenotype during development. To examine whether maternal fish oil supplementation during lactation could modify later immune responses in children, 122 lactating Danish mothers with a fish intake below the population median were randomized to groups supplemented for the first 4 mon of lactation with 4.5 g/d of fish oil (equivalent to 1.5 g/d of n-3 long-chain PUFA) or olive oil. Fifty-three mothers with a fish intake in the highest quartile of the population were also included. The FA composition of erythrocyte membranes was measured at 4 mon and at 21/2 yr. Plasma immunoglobulin E (IgE) levels and cytokine production in lipopolysaccharide-stimulated whole-blood cultures were determined at 21/2 yr. Erythrocyte n-3 PUFA at 4 mon were higher in infants from the fish oil group compared with the olive oil group (P < 0.001) but were no longer different at 21/2 yr. The median production of lipopolysaccharide-induced interferon gamma (IFN-gamma) in the fish oil group was fourfold higher than that in the olive oil group (P=0.034), whereas interleukin-10 (IL-10) production was similar. The IFN-gamma/IL-10 ratio was twofold higher in the fish oil group (P=0.019) and was positively correlated with 20:5n-3/20:4n-6 in erythrocytes at 4 mon (P=0.050). The percentages of atopic children and plasma IgE were not different in the two groups, but the study was not designed to look at atopy. Cytokine responses and erythrocyte FA composition in children of mothers with a high fish intake were intermediate in comparison with those in the randomized groups. Fish oil supplementation during lactation resulted in increased in vitro IFN-gamma production in the children 2 yr after the supplementation was given, which may reflect a faster maturation of the immune system.