Trichinella spiralis:: Vascular endothelial growth factor is up-regulated within the nurse cell during the early phase of its formation
Trichinella spiralis:: Vascular endothelial growth factor is up-regulated within the nurse cell during the early phase of its formation
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DOI：
10.2307/3284472
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发表时间：
1998-04-01
影响因子：
1.3
通讯作者：
Polvere, RI
中科院分区：
文献类型：
--
作者：
Capó, VA;Despommier, DD;Polvere, RI
旋毛虫L1幼虫期诱导部分骨骼肌细胞发生改变，形成滋养细胞。这种特殊的宿主细胞完全包裹在主要由IV型和VI型胶原蛋白组成的胶囊中。其依次被独特的血管网包围，血管网在细胞内感染后约12天开始形成。我们调查的可能性，血管内皮生长因子（VEGF）可能是上调护士细胞形成过程中采用免疫组织化学和原位杂交同步感染的小鼠肌肉组织。从感染后第7天到16个月，在发育中的滋养细胞胞质中检测到VEGF mRNA和VEGF肽。此外，在第15天和第17天，在紧邻保育细胞周围区域的细胞中也检测到VEGF。在此基础上，我们提出T.在VEGF上调之前的某个时间，可能早在第7天，在发育中的滋养细胞内存在螺旋。我们进一步提出，在营养细胞胞质中VEGF持续存在的基础上，维持缺氧细胞的恒定状态。
The L1 larval stage of Trichinella spiralis induces modification in a portion of striated skeletal muscle cell resulting in the formation of the nurse cell. This specialized host cell is completely encased in a capsule composed mainly of collagen type IV and type VI. which, in turn, is surrounded by a unique rete of vessels whose formation begins on around day 12 after intracellular infection. We investigated the possibility that vascular endothelial growth factor (VEGF) may be up-regulated during nurse cell formation by employing immunohistochemistry and in situ hybridization on synchronously infected mouse muscle tissue. Both VEGF mRNA and VEGF peptide were detected in the developing nurse cell cytoplasm from day 7 up to 16 mo after infection. In addition, VEGF was also detected in cells in the area immediately surrounding the nurse cell on days 15 and 17. On the basis of these results, we propose that hypoxia is induced by T. spiralis within the developing nurse cell some time prior to the up-regulation of VEGF perhaps as early as day 7. We further propose, on the basis of the continued presence of VEGF in nurse cell cytoplasm, that a constant state of hypoxia cell is maintained.