Inhibition of aortic intimal hyperplasia and cell cycle protein and extracellular matrix protein expressions by BuYang HuanWu Decoction
Inhibition of aortic intimal hyperplasia and cell cycle protein and extracellular matrix protein expressions by BuYang HuanWu Decoction
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补阳还五汤抑制主动脉内膜增生及细胞周期蛋白和细胞外基质蛋白表达
DOI：
10.1016/j.jep.2009.07.022
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发表时间：
2009-09-25
影响因子：
5.4
通讯作者：
Deng, Chang-Qing
中科院分区：
文献类型：
--
作者：
Wu, Lu;Zhang, Wei;Deng, Chang-Qing
研究目的：补阳还五汤及其主要成分对血管内膜增生及细胞周期蛋白和细胞外基质蛋白表达的抑制作用材料与方法：SD大鼠随机分为假手术组、对照组、生物碱组、苷组、补阳还五汤组和阿托伐他汀组。除假手术组外，其余各组均采用国产球囊导管插入大鼠主动脉内膜损伤。从血管损伤后第二天开始口服药物，持续14天。术后第16天取伤段股骨头，观察血管结构的形态学变化，并检测血管细胞增殖细胞核抗原（PCNA）、细胞周期蛋白D（1）、细胞周期蛋白E（cyclinE）和细胞外基质（ECM）蛋白I型胶原（Col-1）、纤维连接蛋白（FN）的表达。进一步揭示其生物活性物质及可能的作用机制。补阳还五汤总生物碱和总苷抑制内膜增生及PCNA、cyclinD（1）、cychnE、Col-I和FN表达的作用均优于补阳还五汤。提示生物碱和苷类成分可能是补阳还五汤抑制血管内膜过度增生的主要成分。补阳还五汤及其有效成分的抗增殖作用机制可能与阻断VSMC细胞周期有关，抑制ECM蛋白的合成，甚至增加ECM蛋白的降解。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Aim of the study: The inhibitive effect of BuYang HuanWu Decoction (BYHWD) and its major components on vascular intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix protein.Materials and methods: Sprague-Dawley rats were randomly divided into sham-operated, control, alkaloid, glycoside, BYHWD and atorvastatin groups. Rat aorta intima in all groups were injured by insesion of domestic balloon catheter into the aortae except sham-operated rats. Drugs were administrated orally from the second day after vascular injury and continued for 14 days. The injured segments of aortae were collected on the sixteenth day after operation to observe the morphological changes of vascular structure and to examine the expressions of proteins in vascular cells associated with cell cycle including proliferating cell nuclear antigen(PCNA), cyclinD(1) and cyclinE, and extracellular matrix(ECM) proteins including collagen I (Col-1) and fibronectin (FN), further to discover the involved biologically active substances and the potential mechanisms.Results: Alkaloid and glycosid isolated from BYHWD were more effective than BYHVVD in the inhibition of intimal hyperplasia and the expressions of PCNA, cyclinD(1), cychnE, Col-I and FN, suggesting that alkaloid and glycoside may be the main components of BYHWD responsible for the observed inhibition of excessive hyperplasia of vascular intima.Conclusions: The mechanism associated with the anti-hyperplasia activity of BYHVVD and its effective components may be related to the blockage of cell cycles of VSMC, and the inhibition of the ECM protein synthesis, even the increased degradation of ECM proteins. (C) 2009 Elsevier Ireland Ltd. All rights reserved.