Clathrin-dependent endocytosis predominantly mediates protein absorption by fat body from the hemolymph in Bombyx mori
Clathrin-dependent endocytosis predominantly mediates protein absorption by fat body from the hemolymph in Bombyx mori
复制标题
网格蛋白依赖性内吞作用主要介导家蚕脂肪体从血淋巴中吸收蛋白质
DOI：
10.1111/1744-7917.12674
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发表时间：
2019
期刊：
影响因子：
4
通讯作者：
Ling Tian
中科院分区：
文献类型：
--
作者：
Yu-Jie Wang;Shu-Yan Li;Jia-Ye Zhao;Kang Li;Jing Xu;Xian-Yin Xu;Wen-Mei Wu;Rong Yang;Yang Xiao;Ming-Qiang Ye;Ji-Ping Liu;Yang-Jin Zhong;Yang Cao;Hui-Yu Yi;Ling Tian
在昆虫幼虫-蛹变态期间，血淋巴中的蛋白质被脂肪体吸收以维持细胞内稳态;然而，蛋白质的类型以及这些蛋白质如何内化到脂肪体中尚不清楚。在家蚕中，血淋巴和脂肪体中总蛋白的发育谱显示，血淋巴减少的蛋白条带（55-100 kDa）与脂肪体中增加的蛋白条带一致。对网格蛋白依赖性内吞作用的抑制主要阻断了55-100 kDa蛋白从血淋巴转运至脂肪体，这通过Bmclathrin的RNA干扰处理进一步验证。通过质谱（MS）分析，六种六聚体蛋白占血淋巴和脂肪体中总鉴定蛋白的约90%。此外，血淋巴特异性蛋白主要参与物质运输，而脂肪体特异性蛋白特别参与代谢。本文首次发现了四种六聚体，并鉴定了脂肪体通过网格蛋白依赖性内吞作用从血淋巴中吸收的潜在蛋白质。本研究为昆虫变态过程中蛋白质的吸收机制提供了理论依据。
During insect larval–pupal metamorphosis, proteins in the hemolymph are absorbed by the fat body for the maintenance of intracellular homeostasis; however, the type of proteins and how these proteins are internalized into the fat body are unclear. InBombyx mori, the developmental profiles of total proteins in the hemolymph and fat body showed that hemolymph‐decreased protein bands (55–100 kDa) were in accordance with those protein bands that increased in the fat body. Inhibition of clathrin‐dependent endocytosis predominantly blocked the transportation of 55–100 kDa proteins from the hemolymph into the fat body, which was further verified by RNA interference treatment ofBmclathrin. Six hexamerins were shown to comprise ∼90% of the total identified proteins in both the hemolymph and fat body by mass spectrum (MS) analysis. In addition, hemolymph‐specific proteins were mainly involved in material transportation, while fat body‐specific proteins particularly participated in metabolism. In this paper, four hexamerins were found for the first time, and potential proteins absorbed by the fat body from the hemolymph through clathrin‐dependent endocytosis were identified. This study sheds light on the protein absorption mechanism during insect metamorphosis.