Adipose Tissue-Secreted Factors Alter Bladder Cancer Cell Migration.
Adipose Tissue-Secreted Factors Alter Bladder Cancer Cell Migration.
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DOI：
10.1155/2018/9247864
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发表时间：
2018
影响因子：
3.3
通讯作者：
Johnson-Pais TL
中科院分区：
文献类型：
--
作者：
Hariharan N;Ashcraft KA;Svatek RS;Livi CB;Wilson D;Kaushik D;Leach RJ;Johnson-Pais TL
肥胖与膀胱癌复发风险增加有关。这项研究调查了脂肪组织在膀胱癌进展中的作用。 对正常体重（n=5）、超重（n=11）和肥胖（n=10）浸润性膀胱癌患者收集的脂肪组织进行基因表达谱分析，并从两名正常体重、两名超重和两名肥胖患者中获得脂肪基质细胞（ASC）。表征并评估了条件培养基 (CM) 对 T24 膀胱癌细胞增殖、迁移和侵袭潜力的影响。 表达谱证明了仓库特异性或体重指数特异性差异。使用从所有 ASC 收获的 CM 观察到 T24 细胞迁移增加。与从正常体重和超重患者收集的 ASC CM 相比，来自肥胖患者的 ASC CM 显着增加了 T24 细胞迁移和侵袭。我们发现 CXCL1、PAI1、IL6、CX3CL1 和 CCL2 在所有 CM 中大量表达。用 PAI1、IL6 和 CXCL1 对 T24 细胞进行外源处理可增强迁移。肥胖患者 ASC CM 中 CXCL1、PAI1 和 IL6 的消耗消除了 T24 迁移。 脂肪组织分泌的因子影响膀胱肿瘤细胞的迁移，并可能在肿瘤进展中发挥积极作用。
Obesity is associated with an increased risk of bladder cancer recurrence. This study investigated the role of adipose tissue in bladder cancer progression. Gene expression profiling was performed on adipose tissues collected from normal weight (n=5), overweight (n=11), and obese (n=10) patients with invasive bladder cancer, and adipose stromal cells (ASCs) were obtained from two normal weight, two overweight, and two obese patients. Conditioned media (CM) was characterized and evaluated for its effects on the proliferation, migration, and invasive potential of T24 bladder cancer cells. Expression profiling demonstrated depot-specific or body mass index-specific differences. Increased T24 cell migration was observed using CM harvested from all ASCs. ASC CM from an obese patient significantly increased T24 cell migration and invasion compared to ASC CM collected from normal weight and overweight patients. We identified abundant expression of CXCL1, PAI1, IL6, CX3CL1, and CCL2 in all CM. Exogenous treatment of T24 cells with PAI1, IL6, and CXCL1 enhanced migration. Depletion of CXCL1, PAI1, and IL6 in an obese patient ASC CM abrogated T24 migration. Factors secreted by adipose tissue influence the migration of bladder tumor cells and could play an active role in tumor progression.