The role of M cells of human nasopharyngeal lymphoid tissue in influenza virus sampling
The role of M cells of human nasopharyngeal lymphoid tissue in influenza virus sampling
复制标题
DOI：
10.1007/s00428-003-0898-8
复制
发表时间：
2004-01-01
期刊：
影响因子：
3.5
通讯作者：
Ohuchi, M
中科院分区：
文献类型：
--
作者：
Fujimura, Y;Takeda, M;Ohuchi, M
关于人鼻咽淋巴组织的M细胞在引起呼吸道感染的病毒取样中的作用，人们知之甚少。为了阐明M细胞是否可以作为流感病毒进入人鼻咽淋巴组织的通道，将切除的腺样组织分别在含有甲型流感病毒的培养基中孵育30、60和90分钟。透射电镜观察发现，许多流感病毒粘附在M细胞表面，并采取了到M细胞的胞质囊泡孵育30分钟后，病毒已被运送到包裹淋巴细胞孵育60分钟后。通过用特异性识别唾液蛋白的NeuAcalpha2，6 Gal连接的黑接骨木凝集素对M细胞染色，还发现M细胞表面上存在丰富的人流感病毒受体。我们的研究结果表明，人鼻咽扁桃体的M细胞作为一个主要的端口，流感病毒进入，并可以有效地转移到M细胞的包裹的巨噬细胞和淋巴细胞。M细胞将流感病毒转运至淋巴样细胞可能促进抗原递送至免疫系统，这些发现可能对于那些具有生产性感染呼吸道外细胞能力的流感病毒的全身递送很重要。
Little is known about the role of the M cells of human nasopharyngeal lymphoid tissue in the sampling of viruses that cause respiratory infections. To clarify whether M cells could function as a gateway for influenza virus into human nasopharyngeal lymphoid tissue, excised adenoid tissue was incubated in media containing influenza A virus for 30, 60, and 90 min, respectively. Transmission electron microscopic observation revealed that many influenza viruses adhered to M cell surfaces and were taken up into the cytoplasmic vesicles of M cells after 30 min incubation; the viruses had been transported into enfolded lymphoid cells after 60 min incubation. By staining M cells with Sambucus nigra lectin, which specifically recognizes the NeuAcalpha2,6 Gal linkage of sialoprotein, it was also found that abundant receptors for the human influenza virus are present on the M cell surface. Our findings indicated that M cells of human nasopharyngeal tonsils function as a major port for influenza A virus entry and that the virus could be efficiently transferred to enfolded macrophages and lymphoid cells by M cells. The transport of influenza viruses to lymphoid cells by M cells may promote antigen delivery to the immune system, and these findings may be important for systemic delivery of those influenza viruses that have the capacity to productively infect cells outside of the respiratory tract.