Morphologic changes in round window membrane after topical hydrocortisone and dexamethasone treatment
Morphologic changes in round window membrane after topical hydrocortisone and dexamethasone treatment
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DOI：
10.1097/00129492-200303000-00034
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发表时间：
2003-03-01
影响因子：
2.1
通讯作者：
Anniko, M
中科院分区：
文献类型：
--
作者：
Nordang, L;Linder, B;Anniko, M
假设：所有糖皮质激素都应该对圆窗膜有相同的作用吗？背景：人们对糖皮质激素局部治疗车内疾病的兴趣正在增加。因此，此类治疗的安全性应成为临床使用前的重要考虑因素。 方法：在这项研究中，作者研究了中耳局部滴注地塞米松或氢化可的松后圆窗膜的形态。使用二十只斯普拉格-道利大鼠。五只大鼠右耳接受 5 剂、五只大鼠 10 剂 1 杯 (20μl) 地塞米松，另外五只大鼠右耳也接受 5 剂、五只大鼠 10 剂 2% (20μl) 氢化可的松溶液。在光学显微镜和透射电子显微镜中研究了膜形态。测量暴露耳膜的厚度并与对照耳膜进行比较。结果：在暴露于氢化可的松的耳膜中观察到增厚和显微镜下的炎症迹象，但在暴露于地塞米松的耳膜中未观察到，其形态与对照耳膜没有差异。结论：虽然氢化可的松具有抗炎特性，但在局部滴注后，它似乎会引起圆窗膜的炎症。然而，地塞米松没有这样的作用。
Hypothesis: Are all glucocorticoids supposed to have the same effect on the round window membrane?Background: Interest in glucocorticoids for topical treatment of inner car diseases is increasing. The safety of such treatment should therefore be an important consideration before clinical use.Methods: In this study the authors investigated the morphology of the round window membrane after topical instillation of dexamethasone or hydrocortisone into the middle ear. Twenty Sprague-Dawley rats were used. Five rats received 5 doses, and five rats 10 doses, of 1 mug (20mul) dexamethasone in the right ear, and five others were given 5 doses, and five rats 10 doses, of 2% (20mul) hydrocortisone solution, also in the right ear. Membrane morphology was studied in both light microscopy and transmission electron microscopy. The thickness of ex posed membranes was measured and compared with that of control membranes.Results: Thickening and microscopically signs of inflammation were observed in hydrocortisone-exposed membranes but not in dexamethasone-exposed membranes, which did not differ morphologically from those in control ears.Conclusion: Although hydrocortisone has anti-inflammatory properties, it seems to provoke inflammation in the round window membrane after topical instillation. Dexamethasone had no such effects, however.