Juvenile hormone as a causal factor for maternal regulation of diapause in a wasp
Juvenile hormone as a causal factor for maternal regulation of diapause in a wasp
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DOI：
10.1016/j.ibmb.2022.103758
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发表时间：
2022-03-16
影响因子：
3.8
通讯作者：
Goto, Shin G.
中科院分区：
文献类型：
--
作者：
Mukai, Ayumu;Mano, Genyu;Goto, Shin G.
大多数温带多化性昆虫进入滞育，一种由植物控制的发育暂停，响应季节性光周期和/或热线索。一些昆虫物种表现出滞育的母性调节，其中后代的发育轨迹由母亲响应于母亲接收的环境线索来决定。虽然昆虫的滞育是由母体调节的，但其母体内分泌机制还很不清楚。为了解决这个问题，我们使用了宝石黄蜂Nasonia vitripennis，它在长日照下产生非滞育后代，在短日照或低温下产生滞育后代。这些黄蜂的比较转录组学揭示可能参与保幼激素（JH）的生物合成级联在孕产妇滞育调节。保幼激素酸性O-甲基转移酶（jhamt）的表达通常在短日黄蜂下调，这是反映了血淋巴JH浓度的减少。针对jhamt的RNAi降低了血淋巴JH浓度，并诱导黄蜂产生滞育后代，即使在长时间的日子。此外，局部应用JH抑制生产滞育注定的后代短日或低温下。这些结果表明，N.丽蝇蛹集茧蜂通过响应于日长的母体Jhamt表达和血淋巴JH浓度来确定。因此，我们报告了一个新的作用，JH在昆虫的季节性。
Most temperate multivoltine insects enter diapause, a hormonally controlled developmental suspension, in response to seasonal photoperiodic and/or thermal cues. Some insect species exhibit maternal regulation of diapause in which developmental trajectories of the offspring are determined by mothers in response to environmental cues that the mother received. Although maternally regulated diapause is common among insects, the maternal endocrinological mechanisms are largely veiled. To approach this issue, we used the jewel wasp Nasonia vitripennis, which produces non-diapause-destined offspring under long days and diapause-destined offspring under short days or low temperatures. Comparative transcriptomics of these wasps revealed possible involvement of the juvenile hormone (JH) biosynthetic cascade in maternal diapause regulation. The expression of juvenile hormone acid O-methyltransferase (jhamt) was typically downregulated in short-day wasps, and this was reflected by a reduction in haemolymph JH concentrations. RNAi targeted at jhamt reduced haemolymph JH concentration and induced wasps to produce diapause-destined offspring even under long days. In addition, topical application of JH suppressed the production of diapause-destined offspring under short days or low temperatures. These results indicate that diapause in N. vitripennis is determined by maternal jhamt expression and haemolymph JH concentration in response to day length. We therefore report a novel role for JH in insect seasonality.