Hemorrhage detection during focused-ultrasound induced blood-brain-barrier opening by using susceptibility-weighted magnetic resonance imaging
Hemorrhage detection during focused-ultrasound induced blood-brain-barrier opening by using susceptibility-weighted magnetic resonance imaging
复制标题
DOI：
10.1016/j.ultrasmedbio.2008.01.011
复制
发表时间：
2008-04-01
影响因子：
2.9
通讯作者：
Wang, Jiun-Jie
中科院分区：
文献类型：
--
作者：
Liu, Hao-Li;Wai, Yau-Yau;Wang, Jiun-Jie
高强度聚焦超声已被发现能够局部和可逆地增加血脑屏障（BBB）的通透性，这可以使用磁共振成像（MRI）检测。然而，也可能发生副作用，如微出血、红细胞外渗或甚至广泛出血。尽管目前的对比增强T1加权MRI可用于检测血脑屏障通透性的变化，但其在检测聚焦超声超声处理后组织出血方面的功效仍然有限。本研究的目的是探讨磁共振磁共振成像（MR-SWI）的可行性，以确定可能的组织出血相关的BBB的破坏引起的聚焦超声在大鼠模型。42只Sprague-Dawley大鼠的大脑接受了107次超声处理，单侧或双侧。通过在微泡存在下将突发模式聚焦超声能量递送到脑组织中来实现局部BBB开放。通过T2加权和对比增强T1加权MRI技术以及SWI对大鼠进行了研究。组织学上的变化进行了分析，并与末端脱氧核苷酸转移酶生物素dUTP缺口末端标记法的凋亡程度进行了研究。结果表明，SWI比标准T2加权和对比增强T1加权MRI技术更敏感地检测脑超声处理后的血管。纵向研究表明，SWI对损伤的恢复过程非常敏感，因此可以为常规MR图像提供重要的补充信息。潜在的应用，如大脑中的药物输送可能会受益。
High-intensity focused ultrasound has been discovered to be able to locally and reversibly increase the permeability of the blood-brain barrier (BBB), which can be detected using magnetic resonance imaging (MRI). However, side effects such as microhemorrhage, erythrocyte extravasations or even extensive hemorrhage may also occur. Although current contrast-enhanced T1-weighted MRI can be used to detect the changes in BBB permeability, its efficacy in detecting tissue hemorrhage after focused-ultrasound sonication remains limited. The purpose of this study is to investigate the feasibility of magnetic resonance susceptibility-weighted imaging (MR-SWI) for identifying possible tissue hemorrhage associated with disruption of the BBB induced by focused ultrasound in a rat model. The brains of 42 Sprague-Dawley rats were subjected to 107 sonications, either unilaterally or bilaterally. Localized BBB opening was achieved by delivering burst-mode focused ultrasound energy into brain tissue in the presence of microbubbles. Rats were studied by T2-weighted and contrast-enhanced T1-weighted MRI techniques, as well as by SWI. Tissue changes were analyzed histologically and the extent of apoptosis was investigated with the terminal deoxynucleotidyl transferase biotin-dUTP nick-end labeling method. The results demonstrated that SWI is more sensitive than standard T2-weighted and contrast-enhanced T1-weighted MRI techniques in detecting hemorrhages after brain sonication. Longitudinal study showed that SWI is sensitive to the recovery process of the damage and, therefore, could provide important and complementary information to the conventional MR images. Potential applications such as drug delivery in the brain might be benefited.