Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.
Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.
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DOI：
10.1016/j.ydbio.2006.01.024
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发表时间：
2006-04
影响因子：
2.7
通讯作者：
Kyoko Miwata;Takuto Chiba;Reiko Horii;Lixy Yamada;A. Kubo;Daisuke Miyamura;N. Satoh;Y. Satou
中科院分区：
文献类型：
--
作者：
Kyoko Miwata;Takuto Chiba;Reiko Horii;Lixy Yamada;A. Kubo;Daisuke Miyamura;N. Satoh;Y. Satou
最近对一些动物基因组的解码提供了关于进化和基因结构的前所未有的信息，但这些信息必须得到精确的基因注释和单个基因的时间和空间表达模式的补充。在本研究中，我们系统地鉴定和鉴定了肠生黄牛基因组中的566个锌指基因，这是一种新兴的全基因组发育和进化研究的模式系统。在这些基因中，有356个基因编码一个潜在的转录因子，该转录因子基于假定的核酸结合活性或未知功能区域。我们进一步研究了225个基因在胚胎发育过程中的表达模式，当考虑到先前的研究时，[Imai，K.S.，Hino，K.，Yagi，K.，Satoh，N.，Satou，Y.，2004。海鞘胚胎转录因子和信号分子的基因表达谱：对基因网络的全面理解。发展131,4047-4058]，我们已经表征了近85%的潜在锌指转录因子的发育表达模式。总体而言，锌指基因在发育过程中优先在母体表达，幼虫表达很少。本研究为该物种全基因组研究提供了有价值的参考，也为进一步研究锌指基因在其他动物中的表达模式提供了有价值的参考。
The recent decoding of a number of animal genomes has provided unprecedented information regarding evolution and gene structures, but this information must be supplemented with precise gene annotations and the temporal and spatial expression patterns of individual genes. In the present study, we systematically identified and characterized 566 zinc finger genes in the genome of Ciona intestinalis, an emerging model system for genome-wide studies of development and evolution. Of these genes, 356 genes encoded a potential transcription factor based on putative nucleic acid binding activity or domains of unknown function. We further examined the expression patterns of 225 genes during embryogenesis, and, when considered with a previous study [Imai, K.S., Hino, K., Yagi, K., Satoh, N., Satou, Y., 2004. Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks. Development 131, 4047-4058], we have characterized the developmental expression patterns of nearly 85% of the potential zinc finger-containing transcription factors. Overall, zinc finger genes are preferentially maternally expressed with little larval expression during development. The present study provides a valuable reference for genome-wide studies in this species and for future studies wishing to examine zinc finger gene expression patterns in other animals.