Effect of honey on antibody production against thymus-dependent and thymus-independent antigens in primary and secondary immune responses
Effect of honey on antibody production against thymus-dependent and thymus-independent antigens in primary and secondary immune responses
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DOI：
10.1089/jmf.2004.7.491
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发表时间：
2004-12-01
影响因子：
2.4
通讯作者：
Haq, A
中科院分区：
文献类型：
--
作者：
Al-Waili, NS;Haq, A
目的是研究天然纯蜂蜜对小鼠产生抗胸腺依赖性抗原[绵羊红细胞（SRBCs）]和胸腺非依赖性抗原（大肠杆菌）抗体的影响。将42只小鼠（平均体重28.33 +/-3.44 g）分成两组：A组（21只小鼠）饲喂常规饮食，和B组（21只小鼠）饲喂常规饮食加0.8 g/kg体重/天的蜂蜜，以四个等分剂量施用。每只动物腹腔注射0.1 mL 5%SRBC和0.1 mL灭活E.杆菌17天后给予相同剂量的两种抗原。在初次免疫后第7天和第16天，以及二次免疫后第4天，在A组和B组前的每个时间间隔采集7只小鼠的血液样品，使用血凝试验估计抗体滴度。初次免疫后第7天，A组和B组抗SRBC抗体滴度分别为9.14 ± 3.02和13.7 ± 3.9（P
The objective was to study the effect of natural pure honey on the antibody production against thymus-dependent antigen [sheep red blood cells (SRBCs)] and thyrnus-independent antigen (Escherichia coli) in mice. Forty-two mice (mean weight 28.33 +/- 3.44 g) were divided into two groups: group A (21 mice) fed regular diet and group B (21 mice) fed regular diet plus 0.8 g/kg of body weight/day of honey administered in four equally divided doses. Each animal was injected intraperitoneally with 0.1 mL of 5% SRBCs and 0.1 mL of killed E. coli. The same dose of both antigens was given after 17 days. At days 7 and 16 after primary immunization and Lit day 4 after secondary immunization, blood samples were collected from seven mice at each time interval front group A and group B to estimate antibody titer using the hemoaggulination test. At day 7 after primary immunization, the mean antibody titer against SRBCs was 9.14 +/- 3.02 in group A and 13.7 +/- 3.9 in group B (P